MORBID ANATOMY 75 



the walls of the pelvis and ureters. The urine is bloody in 

 some instances. 



The vaginal mucus membrane is congested in many cases. 

 Wilson and Brimhall saw one animal that was four months 

 pregnant, which exhibited small areas of hemorrhage in the 

 placental membranes. 



The udder is congested in some cases, in one instance it 

 appeared as one mass of blood. 



The central nervous system was examined in but three 

 cases. The first two showed hemorrhages in the dura. An 

 examination of a small portion of the cord showed no lesions. 

 Two cases showed hemorrhages in all of the joint surfaces. 



Portions of the subcutaneous tissue, skeletal muscles, lym- 

 phatic glands, lung, heart wall, stomach wall, and spleen 

 were fixed in 95 per cent, alcohol and in 4 per cent, formal- 

 dehyde solution and stained by various methods by Wilson and 

 Brimhall. In general, the lesions found were enormous ex- 

 travasations of blood, some recent and some showing coagula- 

 tion of fibrin. In the areas of less recent hemorrhage, the sur- 

 rounding tissues showed varying degrees of ordinary coagula- 

 tion necrosis. This was particularly marked in the affected 

 muscles, lymph glands, and portions of the lungs. In the bor- 

 ders of such necrosed areas leucocytic infiltration was not in- 

 frequent. In the spleen in which the hemorrhagic areas were 

 neither numerous nor large ; there was in some instances, an 

 apparent destruction or shrinkage of the parenchyma. 



A very important feature in this disease has been brought 

 out, namely, that is it necessary, in order to obtain cultures of 

 the bacterium producing it, that the media should be inocu- 

 lated at once. It is evident from the literature that with this 

 precaution cultures should invariabh- be obtained. The writer 

 had occasion to investigate an outbreak of disease of consider- 

 able proportions in Central New York where the lesions cor- 

 responded exactly with those more recently described by Wil. 

 son and Brimhall. Allthough a very large number of cover- 

 glass preparations from the blood and different tissues were 

 made and stained by various solutions and methods, and many 

 tubes of bouillon, agar and gelatin were inoculated, bacteria 

 were not found in the tissues of any of the animals examined. 



