106 THE FUNGAL DISEASE: EMPUSA MUSCAE COHX 



when discharged from the bodies of the flies, bacteria of the colon group. He was 

 unable to cultivate the fungus artificially. From the fact that in the body of the 

 diseased fly the fungal hyphae closely invest the chorion of the eggs he believes 

 that the larvae emerging from such eggs might become infected with the fungus 

 and thus transmit the infection to the next generation. I should point out, how- 

 ever, that there are two strong objections to this possibility. First, once a fly is 

 attacked by the fungus it would be incapable of ovipositing by the time the disease 

 had reached a stage where the ovaries have become infected with the fungus. 

 Secondly, even did the first objection not exist, the emergence of the larva from 

 the egg is of such a nature as to permit the larva to escape infection, especially as 

 the fungal hyphae do not penetrate the chorion of the egg. 



Buchanan records the fact that Morgan has succeeded in obtaining an artificial 

 culture of the fungus (see Brit. Med. Journ. Jan. 4th, 1913). 



