viii CONTENTS. 



CHAPTER VI. 



Preparation of the tubes, flasks, etc., in which the media 

 are to be preserved 124-127 



CHAPTER VII. 



Technique of isolating bacteria in pure culture by the 

 plate and the tube methods 128-135 



CHAPTER VIII. 



The incubating-oven Safety burner employed in heating 

 the incubator Thermo-regulator Gas- pressure regu- 

 lator 136-143 



CHAPTER IX. 



The study of colonies Their naked-eye peculiarities and 

 their appearance under different conditions Differ- 

 ences in the structure of colonies from different species 

 of bacteria Stab-cultures Slant-cultures 144-150 



CHAPTER X. 



Methods of staining Cover-slip preparations Impres- 

 sion cover-slip preparations Solutions employed 

 Preparation and staining of cover-slips Staining solu- 

 tions Special staining-methods 151-172 



CHAPTER XI. 



Systematic study of an organism Points to be considered 

 in determining the morphologic and biologic characters 

 of a culture Methods by which the various biologic 

 and chemical characters of a culture may be ascer- 

 tained Facts necessary to permit the identification of 

 an organism as a definite species 173-209 



CHAPTER XII. 



Inoculation of animals Subcutaneous inoculation Intra- 

 venous injection Inoculation into the lymphatic circu- 

 lation Inoculation into the great serous cavities and 

 into the anterior chamber of the eye Observation of 

 animals after inoculation 210-232 



CHAPTER XIII. 



Post-mortem examination of animals Bacteriological ex- 

 amination of the tissues Disposal of tissues and disin- 

 fection of instruments after the examination Study 

 of tissues and exudates during life 233-242 



