CONTENTS 



INTRODUCTION. 



PAGES 



" Omne vivum ex vivo "The overthrow of the doctrine 

 of spontaneous generation Earlier bacteriological stud- 

 iesThe birth of modern bacteriology 17-30 



CHAPTER I. 



Definition of bacteria Difference between parasites and 

 saprophytes Their place in nature Bacterial enzymes 

 Products of bacteria Nutrition of Bacteria Their 

 relation to oxygen Influence of temperature upon their 

 growth Chemotaxis 31-48 



CAAPTER II. 



Morphology of bacteria Chemical composition of bac- 

 teria Mode of multiplication Spore-formation Mo- 

 tility 49-62 



CHAPTER III. 



Principles of sterilization by heat Methods employed 

 Discontinued sterilization Fractional sterilization 

 Apparatus employed Sterilization under pressure 

 Sterilization by hot air Chemical disinfection and steri- 

 lization Thermal death point of bacteria Mode of 

 action of disinfectants Practical disinfection 63-91 



CHAPTER IV. 



Principles involved in the methods of isolation of bac- 

 teria in pure culture by the plate method of Koch 

 Materials employed 92-98 



CHAPTER V. 



Preparation of nutrient media Bouillon, gelatin, agar- 

 agar, potato, blood-serum, blood-serum from small ani- 

 mals, milk, litmus-whey milk, Dunham's peptone solu- 

 tion, lactose litmus-agar, Loeffler's blood-serum mix- 

 ture, the serum-water mixture of Hiss, Guarnixsi's 



gelatin-agar mixture 99-123 



vii 



