SPECIAL MEDIA. 123 



Meat infusion 950 cc. 



Sodium chloride 5 grams. 



Peptone 25-30 grams. 



Gelatin 40-60 grams. 



Agar-agar 8to4 grams. 



Water 50 c.c. 



The agar-agar should be completely dissolved sepa- 

 rately in about 100 c.c. of water in the autoclave while 

 the other ingredients are being prepared. The latter 

 should be filtered and the dissolved agar-agar added to 

 the nitrate. 



A complete list of the special media would be too 

 voluminous for a book of this size. For their descrip- 

 tion the reader is referred to the current literature. 

 Those that have been given above will suffice for ob- 

 taining a clear understanding of the principles of the 

 subject. In the chapters upon the Pathogenic Bacteria 

 such special media as have proved of use for purposes 

 of identification and differentiation are described in 

 detail. 



