216 BACTERIOLOGY. 



mals is to be taken. The manipulation can easily be 

 made without the aid of an assistant. Its construction 

 is seen in Fig. 42. 1 



For ordinary subcutaneous inoculations at the root of 

 the tail in mice a simple apparatus consists of a piece of 

 board about 7 x 10 cm. and 2 cm. thick, upon which is 

 tacked a hollow truncated cone of wire gauze, about 6 

 cm. long and about 1.5 cm. in diameter at one end and 

 2 cm. at its other end. This is tacked upon the board 



FIG. 43. 



Mouse-holder, with mouse in proper position. 



in such a position that its long axis is in the long axis 

 of the board, being equidistant from its sides. Its small 

 end is placed at the edge of the board. The mouse is 

 taken up by the tail by means of a pair of tongs and 

 allowed to crawl into the smaller end of the wire cone. 

 When so far in that only the root of the tail projects 

 the animal is fixed in this position by a clamp and 

 thumb-screw, with which the apparatus (Fig. 43) is 

 provided. The animal usually remains perfectly quiet 

 and may be handled without difficulty. 



The hair over the root of the tail is to be care- 

 fully cut away with scissors and a pocket cut through 

 the skin at this point. The inoculation is then made 



i Cenlralblatt fur Bacteriologie and Parasitenkunde, 1895, vol. xviii. 

 p. 580. 



