INOCULATION INTO LYMPHATIC CIRCULATION. 223 



stance of the testicle and then injects the amount desired. 

 Injections made in this manner are usually followed 

 by instructive pathological lesions of the lymphatic 

 apparatus of the abdomen. 



INOCULATION INTO THE GREAT SEROUS CAVITIES. 

 Inoculation into the peritoneum presents no difficulties 

 if fluids are to be introduced. In this case one makes, 

 with a pair of sterilized scissors, a small nick through 

 the skin down to the underlying fasciae, and, taking a 

 fold of the abdominal wall between the fingers, plunges 

 the hypodermic needle through the opening just made 

 directly into the peritoneal cavity. There is little or no 

 danger of penetrating the intestines or other internal 

 viscera if the puncture be made along the median line 

 at about midway between the end of the sternum and 

 the symphysis pubis. Though this may seem a rude 

 method, it is rare that the intestines are penetrated or 

 otherwise injured. The object of the primary incision 

 is to lessen the chances of contamination by bacteria 

 located in the skin, some of which might adhere to the 

 needle if it were plunged directly through the skin, and 

 thus complicate the results. 



If solid substances, bits of tissue, etc., are to be intro- 

 duced into the peritoneum, it becomes necessary to con- 

 duct the operation under an anesthetic and upon the 

 lines of a laparotomy. The hair should be shaved from 

 a small area over the median line, after which the skin 

 is to be thoroughly washed. A short longitudinal 

 incision (about 2 cm. long) is then to be made in the 

 median line through the skin and down to the fascia?. 

 Two subcutaneous sutures, as employed by Halsted, are 

 then to be introduced transversely to the line of incision 

 about 1 cm. apart, and their ends left loose. This 

 particular sort of suture does not pass through the skin, 



