302 BACTERIOLOGY. 



In horses toxic symptoms are often the conspicuous 

 result of this mode of inoculation. 



The only successful attempts to reproduce the morbid 

 conditions from which the organism is obtained are 

 those in which the living cultures have been injected 

 directly into the meninges. Weichselbaum produced 

 congestion with pus formation in the meninges of dogs 

 and rabbits by direct injection through openings made 

 in the skulls ; Councilman, Mallory, and Wright caused 

 the death of a goat by the injection into the spinal canal 

 of 1 c.c. of a bouillon suspension of a pure culture of 

 the organism, the autopsy revealing intense congestion 

 of the meninges of both brain and cord, with slight 

 clouding of the meninges and slight increase of menin- 

 geal fluid, and Flexner 1 succeeded, through injections 

 of cultures into the spinal canal of monkeys, in causing 

 death of the animals with inflammation of the meninges 

 of the cord and brain. 



While the portal of entry for this organism to the 

 system is not known, it is still of importance to note 

 that it often makes its exit from the body by way of the 

 organs that are secondarily involved, and that open to 

 without, as the ear, nose, eye, and lungs. 



It is of equal importance to note that the organism is 

 of very low power of resistance, being destroyed in 

 twenty-four hours by direct sunlight and by drying at 

 body-temperature, and in seventy-two hours by drying 

 in the dark at ordinary room-temperature. 



For the diagnosis of epidemic cerebro-spinal menin- 



i Flexner : Jour. Exp. Med. 1907, vol. Ix, p. 168. 



