336 7U CTERIOLOG Y. 



human body, lii the influenza patient it is present in 

 the catarrhal secretions, bronchial mucous membrane, 

 and the diseased lung-tissues. It may be demonstrated 

 microscopically in the mucus by cover-slip prepara- 

 tions made in the usual way and stained with diluted 

 carbol-fuchsin, referred to above. In the tissues it 

 may be demonstrated in sections stained in the same 

 solution. In the sputum the bacteria are found as 

 masses and as scattered cells. (See Fig. 63.) They are 

 also found within the bodies of leucocytes, especially in 

 the later stages of the disease when convalescence has 

 set in; at this time they appear as very small, irregular, 

 evidently degenerated bacteria within white blood- 

 corpuscles. They are also present in the nasal secre- 

 tions. 



At autopsies it is advisable to cut out pieces of 

 the diseased tissue about the size of a pea or a bean, 

 break them up in a small quantity of sterile water or 

 bouillon, and make the cultures from this infusion. 

 By this procedure two advantages are gained : first, 

 a dilution of the number of bacteria present; and, 

 secondly, the tissue furnishes the amount of hemo- 

 globin necessary for the growth of the organism. 

 Under these circumstances it is, of course, not neces- 

 sary to make a further addition of blood to the culture- 

 medium. 



The only animal that has been found susceptible 

 to inoculation with this organism is the monkey. By 

 intratracheal injection Pfeiffer succeeded in causing a 

 toxic condition that proved fatal. He does not regard 

 the death of the animals as due to infection, but rather 

 to intoxication. The disease, as seen in man, has not 

 been reproduced in animals. 



