360 BACTERIOLOGY. 



usually negative. Occasionally the superficial lymphatic 

 glands in the neighborhood of the site of inoculation 

 may be inflamed and purulent. This we have seen only 

 with the subcutaneous inoculation. If pure cultures be 

 injected into the peritoneal cavity along with some 

 sterile, irritating substance, such as sterilized butter, a 

 widespread fibrinopurulent peritonitis is commonly the 

 result. 



When injected directly into the circulation of rabbits, 

 the kidneys are almost uniformly affected, and in the 

 majority of instances they are, singularly enough, the 

 only organs in which lesions are to be detected. If, for 

 instance, a cubic centimetre of a carefully prepared 

 suspension in bouillon of, let us say, Holler's grass 

 bacillus II., be injected into the circulation of a rabbit, 

 and the animal be killed after twelve to fourteen days, 

 the kidneys will be found marked by gray or yellowish 

 points that range in size from that of a pin-point to 

 that of a pin-head. They are sometimes very few in 

 number, but in other cases both kidneys may be thickly 

 studded with them. Often they are not elevated above 

 the cortex of the organ, but in as many cases they are 

 sharply defined, yellow in color, and stand up promi- 

 nently from the cortical surface, being at the same time 

 so adherent to the capsule that the removal of the latter 

 tears them out bodily from the substance of the organ. 

 In the very early stages of development these nodules 

 are often difficult to distinguish from young tubercles, the 

 reaction of the tissues being, as in the case of tubercles, 

 characterized by proliferation of the fixed cells with 

 little evidence of leucocytic invasion ; later on, true 

 giant-cell formation is recorded by some observers. We 

 have not seen this. Clumps of endothelial nuclei or of 



