386 BA CTEEIOL OO Y. 



horse -serum in a dilution of 1 to 300 : 1 to 200 causes the 

 agglutination, while that from glanders animals does the 

 same in from 1 to 3200 to 1 to 500 dilution. 



MALLEIN. The filtered products of growth of the 

 glanders bacillus in fluid media represent what is known 

 as mallein a solution of compounds that bear to 

 glanders a relation analogous to that which tuberculin 

 bears to tuberculosis. It is used with considerable suc- 

 cess as a diagnostic aid in detecting the existence or 

 absence of deep-seated manifestations of the disease, the 

 glanderous animal reacting (manifested by elevations of 

 body-temperature greater than 1.5 C.) to subcutaneous 

 injections of mallein in from four to ten hours, while an 

 animal not so affected gives no such reactions. 



Mallein is prepared from old glycerin-bouillon cult- 

 ures of the glanders bacterium by steaming them for 

 several hours in the sterilizer, after which they are fil- 

 tered through unglazed porcelain. 



By some it is said that the repeated injection of mal- 

 lein in small doses confers immunity from infection by 

 bacterium mallei upon animals so treated ; an opinion 

 that is entirely in accord with the principles underlying 

 the artificial induction of immunity in general. 



