488 BACTERIOLOGY. 



growth is essentially that of microspira comma, though 

 at times it is a little more rapid in progress. 



On meat-infusion agar-agar, neutral or slightly alka- 

 line to phenolphthalein, growth is very rapid at the 

 body temperature. The general character of the growth 

 corresponds to that of microspira comma. 



The growth on blood serum, after twenty-four hours 

 at body temperature, appears as a line of depression, 

 which increases as a track of liquefaction, and later re- 

 sults in the more or less complete liquefaction of the 

 medium. 



Bouillon becomes uniformly clouded in twenty-four 

 hours at the body temperature. Its reaction becomes 

 more alkaline as growth progresses. A pellicle, at first 

 delicate, later denser, always characterizes the growth 

 in this medium. 



Usually no visible growth occurs on a potato. 



In fresh litmus milk a slight degree of acidity is no- 

 ticed after twenty-four hours at body temperature. After 

 forty-eight hours this acidity is slightly greater, and at 

 times the milk shows evidences of coagulation, though 

 not always. 



Microspira Schuylkilliensis is a facultative aerobe. 

 In fluid media under an atmosphere of carbon dioxide 

 in sealed tubes no growth is observed. 



The organism grows most luxuriantly at about 37.5 

 C. Growth is hardly perceptible at 10 C. It is 

 destroyed by an exposure of five minutes to 50 C. 



None of the carbohydrates are broken up with the 

 liberation of gas. 



It produces indol and at the same time reduces 

 nitrates to nitrites. 



