ANTHRAX IMMUNE SERUM. 501 



Pasteur method : (1) That the immunization can be car- 

 ried out without losing any of the animals ; (2) that it 

 can be completed in one day ; (3) that stronger and more 

 active cultures can be employed and therefore a more 

 durable immunity obtained; and (4) that the serum alone 

 can be employed as a curative agent. 



ANTHRAX IMMUNE SERUM. Sanfelice 1 experimented 

 with the serum of dogs that had been immunized from 

 anthrax bacteria. This serum possessed immunizing 

 and curative properties, as shown by experiments upon 

 animals. He had an opportunity of trying the serum, 

 with favorable results, upon a man who had contracted 

 anthrax. The total amount of serum employed was 56 

 cubic centimetres. There was no reaction at the point 

 of injection of the serum. The therapeutic effect of the 

 administration of serum was a general improvement in 

 the symptoms, marked fall of the temperature on the 

 second, and complete apyrexia on the third, day. The 

 effect on the local anthrax lesion manifested itself in 

 reduction and, finally, disappearance of the oedema, fol- 

 lowed first by an increased swelling of the glands, which 

 decreased again subsequently. He states that the serum 

 treatment should be continued not only till the temper- 

 ature has fallen to normal and a diminution of the 

 oedema is apparent, but also until there is marked re- 

 duction in the size of the swollen lymph-glands. 



Sclavo 2 immunized a number of animals, principally 

 sheep and goats, with the two vaccines of Pasteur, fol- 

 lowed by repeated injections of increasing quantities of 

 virulent cultures. By this means he obtained an im- 

 mune serum which had protective as well as curative 

 properties when tested upon guinea-pigs and rabbits. 



i Sanfelice : Centralblatt f. Bacteriologie, Originate, 1902, Bd. 38. 

 2 Sclavo: Bulletin de Tlnstitut Pasteur, T. I., 1903. p. 305. 



