502 BA CTERIOL OG Y. 



He found that this serum had neither bactericidal nor 

 antitoxic properties. 



Cicognani 1 employed Sclavo's immune serum on 12 

 persons suffering from various grades of anthrax infec- 

 tion, some of the cases being severe infections in which 

 the prognosis would otherwise have been very unfavor- 

 able. The duration of the disease .was always very 

 much shortened and all recovered. 



Lazaretti 2 reports 23 cases of human infection with 

 bacterium anthracis in which Sclavo's immune serum 

 was employed with recovery in each case. Another 

 patient, suffering from chronic alcoholism and malaria, 

 did not recover. 



EXPERIMENTS. 



Prepare three cultures of bacterium anthracis one 

 upon gelatin, one upon agar-agar, and one upon potato. 

 Allow the gelatin culture to remain at the ordinary 

 temperature of the room, place the agar-agar culture 

 in the incubator, and the potato culture at a temper- 

 ature not above 18 to 20 C. Prepare cover-slips 

 from each from day to day. What differences are 

 observed ? 



Prepare two potato cultures of bacterium anthra- 

 cis. Place one in the incubator and maintain the 

 other at a temperature of from 18 to 20 C. Ex- 

 amine them each day. Do they develop in the same 

 way? 



From a fresh culture of bacterium anthracis, in which 

 spore-formation is not yet begun (which is the surest 



i Cicognani : Centralblatt f. Bacteriologie, 1902, ref. Bd. 31, p. 725. 

 2 Lazaretti: Deutsche Vierteljahrsschrift f. offentliche Gesundheits- 

 pflege, 1903, Bd. 35, Supplement, p. 253. 



