516 



BACTERIOLOGY. 





It grows in ordinary nutrient gelatin and agar-agar 

 of a slightly alkaline reaction. Gelatin is slowly lique- 

 fied, with the coincident production of a small amount 

 of gas. Neither agar-agar nor 

 blood-serum is liquefied by its 

 growth. 



The addition to the media of 

 from 1.5 to 2 per cent, of glucose, 

 0.1 per cent, of indigo-sodium 

 sulphate, or 5 per cent, by volume 

 of blue litmus tincture favors its 

 growth. 



It grows well in alkaline bouil- 

 lon under an atmosphere of hy- 

 drogen. 



Under artificial conditions it 

 may be cultivated through nu- 

 merous generations without loss 

 of virulence. 



APPEARANCE OF THE COLO- 

 NIES. Colonies of bacillus tetani 

 on gelatin under an atmosphere 

 of hydrogen have, in their early 

 stages somewhat the appearance 

 of the colonies of the common 

 bacillus subtilis in their earliest 

 stages, viz., they have a dense, 

 colonies of the tetanus felt-like centre surrounded by a 



iTStMEZTteSf frin g e of delicate radiL The 



isms through a tube nearly liquefaction is SO slow that the 

 filled with glucose-gel- . , . -, f -, 



atin. cultivation in an at- appearance is retained for a rela- 



mosphere of hydrogen, tively long time, but eventually 

 (From FRANKEL and . ', 



PFEIFFEK.) becomes altered. In very old 



