214 SAMUEL C. PRESCOTT 



On being autopsied, each showed a mass of inflamed tissue at the 

 point of inoculation, and from this inflamed portion the bacilli 

 were recovered in large numbers. 



As a second test two guinea-pigs were inoculated subcutane- 

 ously with i c.c. of the same cultures as were used in the previous 

 experiment. In this experiment the animals appeared dull, and 

 their temperature fell slightly in six hours, while at the end of 24 

 hours the temperature had risen above normal, and the animals 

 were decidedly sick and weak, and showed swelling at the point of 

 inoculation. At the end of 48 hours the temperature had still further 

 risen, but no further change was noticeable. One of these animals 

 was autopsied, the other was kept, and ultimately fully recovered. 



A third pair of animals was inoculated intraperitoneally with 

 i c.c. of cultures of B. acidi lactici and a culture from feces respec- 

 tively. In this case the animals showed much stronger lesions, 

 although death was not caused in 48 hours. As in the previously 

 described experiments, no difference could be observed in the beha- 

 vior of the cultures from the two sources. On chloroforming the 

 animals, cultures taken from peritoneum and heart's blood showed 

 the presence of pure cultures of the germs used for inoculation. 



Two more animals were infected intraperitoneally, one with 

 another culture of B. acidi lactici, and the other with another culture 

 from feces. In this experiment the amount of the dose was 1.5 c.c. 

 in each case. A sharp temperature drop was observed in six hours, 

 and in 24 hours each of the animals was dead. Cultures made 

 from blood and peritoneum gave a pure growth of B. coll. 



These experiments show conclusively that the pathogenic power 

 of the organisms derived from grain is fully as great as with the 

 intestinal or more nearly parasitic colon bacilli, and, we believe, 

 offers a strong support to the proof of their actual identity. Some 

 more recent work by Gordan 24 has shown the pathogenic properties 

 for white mice of organisms derived from bran. 



In all investigations thus far reported some doubt might be cast 

 on the integrity of the samples, or at least there is a possibility of 

 contamination from handling or manufacture. To eliminate this 

 objection, the following work was carried out during the late autumn 

 and winter of 1904. In November a field of rye was found by one 



