

STAINS, ETC. 



1379 



heat ; add the glycerin. Mix and filter through the best 

 lish filter-paper, on which has been deposited a 

 thin layer of talc. Glycerin and Formic Acid. 

 This is especially used for picrocarmin preparations, 

 and i? made by adding formic acid to dilute glycerin — 

 I per cent. Goadby's Fluids. First formula : Bay- 

 salt i coarse sea-salt) 3 iv, alum 5 ij, mercuric chlorid 

 - . boiling water I qt. This fluid, diluted with an 

 equal volume of water, is recommended by Schultze for 

 preserving Medusa:, Echinodermata, Entomostraca, 

 thalmia, Polycystina, and Annelid Larva. He ad- 

 3 the subsequent use of glycerin to secure trans- 

 parency. Second formula : Bay-salt, ^viij, mercuric 

 chlorid gr. 2, water I qt. The absence of the alum 

 renders this fluid suitable for the preservation of objects 

 containing calcium carbonate. Hantschs Liquid. 

 Glycerin 1 part, alcohol 3 parts, water 2 parts. Hart- 

 ing's Fluid. One part of mercuric chlorid to 200 to 

 400 of water. It is recommended for preserving blood- 

 corpuscles, nerve-tissue and muscle-tissue, etc. 

 Hayem's Mounting-media. These are solutions of 

 gum with chloral, for objects stained with carmin or 

 hematoxylin, and of gum with potassium acetate, for 

 objects stained with anilin colors, and are prepared as 

 follows : Fill a tall 60 c.c. glass two-thirds full with gum 

 arabic, and add either a solution of chloral containing 5 

 to 10 per cent, of glycerin or of potassium or ammonium 

 acetate. The gum with frequent shaking dissolves in a 

 few days, and the resulting syrupy fluid is then filtered. 

 Iodin. Liquor iodi (Br. Ph. ) 100 c.c, distilled water 

 300 c.c, glycerin 200 c.c, gum arabic 260 gms. Mix 

 and dissolve without heat ; filter through Swedish paper 

 coated with a thin layer of talc. Used in mounting tis- 

 -tained with iodin. Jaeger's Liquid. Glycerin and 

 alcohol each I part, sea-water 10 parts. Kaiser's Gly- 

 cerin-jelly. Soak one part of gelatin for 2 hours in 6 

 pans of distilled water, then add 7 gm. of glycerin and 



11 gm. of carbolic acid for every 100 gm. of the mix- 

 ture. Warm for 10 to 1 5 minutes, stirring constantly 

 until the flakes produced by the acid have disappeared. 

 Pilter while warm through spun glass laid wet in the 

 filter. For use it must be warmed. Klein's Dammar 

 Lac. Dissolve in 2 oz. of turpentine, i l /z oz. of gum 

 dammar, and filter. Dissolve )A oz. of gum mastic in 

 2 oz. of chloroform, and filter. Mix the two solutions 

 and filter again. Langerhans' Gum and Gly- 

 cerin. A modification of Farrant's medium. Gum 

 arabic 5 parts, in water 5 parts; after 12 hours add 

 glycerin 5 parts, and 5 per cent, aqueous solution of 

 carbolic acid 10 parts. Used in preserving marine 

 animals. Lawrence's Glycerin-jelly. Soak a 

 quantity of Nelson's gelatin for 2 or 3 hours in cold 

 water. Decant the superfluous water, and melt the 

 gelatin by the aid of heat. To 8 parts of the gelatin, 

 when it has cooled, but is still fluid, add I part of 

 white of egg ; boil until the albumin coagulates and the 

 gelatin is clear ; filter through flannel, and to 8 parts 

 of the filtrate add 6 parts of a mixture of I part of 

 glycerin and 2 parts of camphor- water. Levulose. 

 This substance is non-crystallizable, and is recom- 

 mended as a preservative 6f carmin and anilin stains 

 ^hematoxylin partially fades in it). Objects may be 

 brought into it from water. The index of refraction is 

 omewhat higher than that of glycerin. May's Fluid, 

 cerin 60 c.c, 1 per cent, arsenic acid 10 c.c, 

 lethyl-alcohol 10 c.c, water 2occ. Methyl-green. 

 Mmoy's Solution. Meyer's Salicylic Vinegar, 

 solution of one part of salicylic acid in 100 parts of 

 Prroligneous acid. For Larva-, Nematodes, etc., add 

 »l the salicylic vinegar to 10 parts of glycerin 

 diluted with twice its volume of water ; for Infusoria, 

 Add 1 pan of the vinegar to 10 parts of glycerin diluted 



EXAMINATION MEDIA 



with four times its volume of water. Noll's Sali- 

 cylic Vinegar and Gum. A mixture of equal parts 

 of Meyer's dilute fluid and Farrant's medium. ■ This 

 mixture does not become turbid and does not dry up. 

 It is an admirable medium for delicate Crustacea and 

 their larvae. Owen's Fluid. Mercuric chlorid 0.014 

 gm., alum 79 gm., salt 137 gm., water 1680 

 gms. Used for preserving soft-bodied animals. 

 Pacini's Fluids. (a) Mercuric chlorid 1 part, 

 sodium chlorid 2 parts, water 200 parts. Especially 

 useful for the blood-corpuscles of cold-blooded animals. 

 (b) Solution a plus two parts of mercuric chlorid. For 

 the examination of the blood-corpuscles of warm- 

 blooded animals, (c) Mercuric chlorid I part, acetic 

 acid 2 parts, water 300 parts. For the examination of the 

 nuclei of animal tissues, (d) Mercuric chlorid I part, 

 sodium chlorid 2 parts , glycerin (25 Beaume) 13 parts, 

 water 1 13 parts. Let the mixture remain undisturbed 

 2 months ; then take for use I part, dilute it with 3 

 parts of water, and filter. This fluid is recommended 

 as a preservative of all delicate tissues, [e) Mercuric 

 chlorid I part, acetic acid 2 parts, glycerin (25 

 Beaume) 43 parts, water 1 15 parts. This mixture is used 

 for the same purposes as the preceding. It is said to 

 preserve the white but to destroy the red corpuscles of 

 the blood, (f) One part of mercuric chlorid in 200 

 of water. This fluid has been used to remove the 

 salt or acid, when necessary, from objects preserved in 

 the preceding fluids. Modifications of the foregoing so- 

 lutions : a. Sublimate I part, sodium chlorid 2 parts, 

 water loo parts. Used for the more vascular tissues of 

 warm-blooded animals, b. Solution a diluted with an 

 equal volume of water ; for similar tissues of cold- 

 blooded animals, c. One part each of sublimate and 

 salt in 300 parts of water ; for pus-corpuscles, d. One 

 part of sublimate in 300 parts of water ; for blood- 

 corpuscles, e. One part each of corrosive sublimate and 

 acetic acid in 300 parts of water ; for demonstrating 

 the nuclei in epithelia, connective tissue, and pus-cor- 

 puscles, f. Solution <» plus 2 parts of acetic acid ; for 

 ligaments, muscles, and nerves, g. Solution e plus 4 

 parts of acetic acid ; for glandular tissues, h. Corrosive 

 sublimate I part, phosphoric acid I part, water 30 parts; 

 for cartilaginous tissues. Picrocarmin. Recom- 

 mended by Ranvier as a medium for the examination 

 of fresh tissues. Camoy finds that cells live in it for 

 a time, but finally become gorged with water and de- 

 teriorate. Potassium Acetate. A mounting-fluid 

 for sections stained with anilin dyes. Dissolve by 

 gentle heat 250 gm. of potassium acetate in IOO c.c. 

 of distilled water. A nearly saturated solution is also 

 employed. Let a drop flow under the cover-glass, and 

 in 24 hours close the mount. Ripart and Petit's Fluid. 

 A preserving medium for delicate, fresh tissues. Its 

 fixing action is enhanced by the addition of a drop of 

 osmic-acid or corrosive-sublimate solution, and it may 

 be used in combination with methyl-green. It is pre- 

 pared by dissolving 0.2 gm. each of copper acetate 

 and crystallized copper chlorid in glacial acetic acid 

 0.5 gm. , camphor- water and distilled water each 50 

 c.c. Seaman's Glycerin -jelly. Dissolve 10 parts 

 of gelatin in water to the consistency of stiff jelly at 

 the temperature of the room ; add 1 part of glycerin 

 and a little camphor- water. Filter through muslin, 

 and add a little alcohol. Stephenson's Mercuric 

 Iodid and Potassium Iodid. A saturated solution of 

 these substances has an index of 1. 680, the highest of 

 any known aqueous fluid. Tissues are well preserved 

 in this medium, but a precipitate forms in it which ruins 

 the preparations. III. Resinous Media. Canada 

 Balsam. Evaporate the balsam in a water-bath to 

 dryness, and dissolve in an equal volume of xylol, 



