TESTS 1485 



TABLE OF TESTS.— Continued. 



TESTS 



Name. 



Use. 



Reagents. 



Reaction. 



Application. 



Knapp's method. 



Volumetric test for 

 sugar in urine. 



Standard alkaline sol. 

 mercuric c y a n i d 

 (mercuric cyanid 10, 

 sodium hydrate [i. 14] 

 100, water up to 7000). 

 40 c.c. — 0.1 sugar. 



Absence of mer- 

 cury in sol. is in- 

 dicated by filter- 

 paper moistened 

 with 1 drop sol., 

 no longer being 

 blackened b y 

 vapor of ammo- 

 nium sulphid. 



Run sugar sol. from 

 buret into hot, 

 standard sol. 



Kossel's test. 



Hypoxanthin. 



(a) Hydrochloric acid. : 

 (b) Zjnc. (c) So-i 

 dium hydrate. 



Ruby-red color. 



Treat with a and b, 

 and add excess of c. 



Kost's test. 



Free hydrochloric (a) 10 per cent. sol. tan- 

 acid in gastric nin. (b) Sol. metbyl- 

 juice. violet. 



Change from vio- Add a, then b. 

 let to blue or 

 green. 



Modi fi cation of 

 Afalr's test; a 

 precipitates the 

 interfering pep- 

 tones. 



Kuehne's test. 



Syntonin. 



Lime-water. 



Soluble in R.; sol. 

 c oa gulat e d , 

 though imper- 

 fectly, by boil- 

 ing.' 



Dissolve in R. and boil. 



Reaction distin- 

 guishes it from 

 other acid-albu- 

 mins and from 

 alkali-albumins. 



Kuelz's test. 



Biliary acids. 



{a) Dil. sol. cane-sugar. 

 (b) Concent, sulphur- 

 ic acid. 



Red, then 

 color. 



violet 



Evaporate substance ; 

 add one drop a, and 

 a few drops b. 



{uelz's test. 



Hydrogen sulpho- 

 cyanid in urine. 



Dil. sol. ferric chlorid, Red color, 

 with a little hydro- 

 chloric acid. 



Dilute R. until same 

 color as urine, then 

 add. 



Not reliable. 



^andoldt's test. 



Carbolic acid. 



Bromin-water. 



White or yellow- Add excess of R. 

 ish-white cloudi- 

 ness or ppt. 



^angley's test. 



Pepsinogen 

 pepsin. 



and 



Sodium carbonate. 



R. destroys pepsin, 

 but has no action 

 on pepsinogen. 



^assaigne's test. j Semen. 



-atschenberger's 

 test. 



Sol. of lead oxid in ! No yellow color, 

 liquor potassae. ( as would occur 



in albuminous 

 i fluids. 



Moisten stain with R. 

 and dry at 68° F. 



If semen diluted 

 with an albumin- 

 ous sec ret ion, 

 yellow color de- 

 velops. 



Ammonia in urine. 



(a) Sat. sol. copper Apply to filtrate 

 sulphate, (b) Barium [ Nessler's test, 

 hydrate. q. v. 



Treat urine with equal 

 vol. a; neutralize 

 with b ; filter. 



ea's (Carey) test. Gelatin. 



Acid sol. mercuric ni- 

 trate. 



Red color. 



Add R. to liquid. 



ea's (Carey) test. Hydrocyanic acid. 



Ammonio-sulphate of Purplish-red color 

 iron 1, uranium ni- or ppt. 

 trate 1, water 240. 



Add 2 drops of liquid 

 on a porcelain slab 

 to 2 drops R. 



effmann's test. 



Urine in potable 

 water. 



Sol. silver nitrate. 



Brown color. 



Add R. 



egal's test. 



Acetone in urine. 



(a) Hydrochloric acid. 

 (b) Sodium nitro- 

 prussid. (c) L i q . 

 potassae. 



's test. 



Red color, which 

 rapidly disap- 

 pears, and gives 

 place to purple 

 on the addition 

 of acetic acid. 



Acidulate with a, dis- 

 til. To distillate add 

 a few drops each b 

 and c. 



Kreatinin strikes 

 similar color, 

 which disappears 

 when acetic acid 

 is added. 



Free acids and 

 acid salts in gas- 

 tric juice. 



Pure calcium carbon- Change of litmus- 

 ate, test paper. 



Acetone in urine. 



Liq. potassae 3.7 c.c, Crystals of iodo- 

 potass. iodid 1.2; form, 

 place in test-tube 

 and boil. 



Mix filtrate in watch- 

 glass with R. ; test 

 with blue litmus; if 

 latter not reddened, 

 acid salts absent ; if 

 red less intense than 

 before, both pres- 

 ent; if red color not 

 altered, free acids 

 absent or much 

 diminished. 



To R. add 3 7C.0 urine, 

 distil, and acidulate 

 with hydrochlor. acid. 

 Ring of phosphates 

 is formed first ; upon 

 this the iodoform is 

 deposited, later 

 sinking to bottom. 



Very delicate ; .002 

 free hydro- 

 chloric, 0.01 lac- 

 tic acid. 



Alcohol, lactic acid, 

 aldehyd, and cer- 

 tain fatty acids 

 also respond. 



