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3% of nitric acid, until everything except the mitotic 

 figure is decolored. Kaiser's Method. A modifica- 

 tion of Weigert's method for medullated nerves: 

 Harden in Miiller's fluid for 2 days; divide the tissue 

 into slices 2 or4 mm. thick and put it into Miiller's fluid 

 for 5 or 6 days; then transfer it into Marchi's fluid 

 2 parts of Miiller's fluid and I c ' c osmic acid). Wash 

 in water, dehydrate, and embed in celloidin. Treat 

 the sections for 5 minutes with liquor ferri sesquichlorid 

 and distilled water each 1 part and 85^ alcohol 3 

 parts. Wash in Weigert's hematoxylin, then stain in 

 fresh hematoxylin for a few minutes; wash in water, 

 differentiate in Pal's fluid, and neutralize in water con- 

 taining a little ammonia. Kanthack's Medium. 1. 

 For the culti%'ation of gonococci : Collect ascitic fluid 

 in a sterilized jar and place it on ice for 24 hours; 

 transfer the supernatant fluid into test-tubes and place 

 in an incubator at 66° C. for 4 hours; inoculate the 

 test-tubes and put them into a beaker half full of dis- 

 tilled water; cover with a glass plate and solidify in 

 the incubator at 33° C. The cultures appear in from 

 24 to 48 hours. 2. For the cultivation of diphtheria 

 bacilli: Add 2 c.c. of 10% potassium hydroxid to 100 

 c.c. of albuminous exudate (of pleurisy, ascites, etc.); 

 boil and add 1.5 'c of agar-agar previously softened 

 in acidulated water; cook in a steam sterilizer until the 

 agar is dissolved ; filter in a hot- water funnel and add 

 4 or 5 % of glycerol to the filtrate ; fill into test-tubes 

 and sterilize. Kantorowicz's Method for staining 

 amyloid substance: Place the sections for 5 minutes in 

 saturated aqueous solution of thionin, wash in distilled 

 water, dehydrate and clear in anilin-xylol (or carbol- 

 xylol), wash in xylol, and mount in xylol-balsam. 

 The amyloid masses are pale blue to lilac, the remain- 

 ing tissues bluish to violet Kemp's Method for 

 blood-platelets : Place a large drop of blood on a slide 

 and quickly wash it with a small stream of normal salt 

 solution. The platelets will adhere to the glass. The 

 preparation will be permanent if, after Eberth and 

 Schimmelbusch, the finger is pricked through a drop 

 of osmic acid. Kiefer's Medium for the cultivation 

 of gonococci : a. Filter acetic fluid, fill into test-tubes, 

 and sterilize fractionally at 62 C. b. Prepare a mix- 

 ture of agar 3.5^, peptone 5%, glycerol 2^, sodium 

 chlorid 0.5^. Liquefy and cool to 50 C. ; mix with 

 an equal volume of a and pour into petri dishes. 

 Kionka's Method for the orientation of avian em- 

 bryos : Open the egg in salt solution, detach the shell 

 and albumen, and locate the poles by thrusting in at a 

 centimeter from the blastoderm two hedgehog spines, 

 marking that at the obtuse end with a red thread. Place 

 in water at 90 C. for 10 minutes, then in 70^ alcohol. 

 After 24 hours dissect out the blastoderm with a little 

 of the yolk in the form of an isosceles triangle the 

 base of which marks the cephalic end of the blastoderm. 

 Kionka's Stain for avian embryos: Dissect a little 

 of the yolk out with the blastoderm. Stain the sections 

 with borax-carmin and wash in acid-alcohol of which 

 each 5 c.c. contains one drop of concentrated solution 

 of orange G, which stains the yolk. Kizer's Method 

 for preserving and staining blood: Mix I volume of 

 blood with 3 volumes of 2% formalin and after an hour 

 pipet a drop of the sediment to a cover-glass; dry, fix 

 by heat, and dip once or twice into a 5 % solution of 

 acetic acid; wash in water and stain in any of the usual 

 hematologic dyes. Klein's Method for the spores of 

 bacteria : Prepare an emulsion of the spore-containing 

 material in o.b r ' c salt solution, add an equal volume of 

 filtered carbol fuchsin and warm gently for 6 minutes. 

 Diffuse the mass, drv by evaporation in the air, fix in 

 the flame and decolor for I or 2 hours in I ^ sulfuric 

 acid. Wash in water and stain in diluted aqueous- 

 32 



alcoholic solution of methylene-blue. Koch's Test. 

 See Silk Thread Test. Kochel's Method for fibrin: 

 Treat sections of tissue hardened in any preferred way 

 for 10 minutes with 1% chromic acid ; wash for a few 

 seconds and then stain for 15 minutes in Weigert's 

 hematoxylin ; wash and treat for a minute with ioff 

 aqueous solution of alum ; rinse and differentiate for 

 about 5 minutes in Weigert's borax-potassium-ferricy- 

 anid diluted with 3 volumes of water; rinse and treat 

 for from a quarter to one hour with io£- alum solu- 

 tion ; rinse and counterstain with cannin or safranin. 

 Kolster's Stain for the differentiation of the gland 

 cells of the stomach : Overstain sections (of material 

 fixed in any medium except osmic acid 1 in hematoxylin, 

 decolor in 1 ( c hydrochloric acid to a faint rose hue, 

 neutralize in 1^ ammonia alcohol until a delicate blue 

 color appears ; wash in distilled water and stain for 

 5 minutes in weak aqueous solution of acid fuchsin ; 

 distilled water ; alcohol ; oil ; balsam. Chief cells 

 pale blue with dark blue nuclei, parietal cells pure 

 fuchsin color with dark nuclei. Kopsch-Golgi 

 Method for ganglion-cells: Place the tissue in freshly 

 prepared mixture of $.$fc potassium bichromate, 4 

 parts, and formalin, I part. Kenew in 1 2 hours and 

 after 24 hours transfer to 3.5^ bichromate minus the 

 formalin, and from this to the silver solution. Korol- 

 ko's for blood : Solution A. A saturated aqueous solu- 

 tion of methylene-blue, 3 months old and filtered before 

 use. Solution B. A I $• aqueous solution of eosm. 

 To make up the stain add to 2 c.c. or 3 c.c. of solution 

 A, from 3 c c. to 5 c.c. of solution B until a violet color 

 is obtained, and a fine granular precipitate is formed. 

 Mix the solutions in a narrow cylinder, and stir with a 

 glass rod. Stain in this mixture 15 to 24 hours, if 

 possible, at a temperature of 30 C. Fix blood-films by 

 heating for I hour at IC5 to I lO° C. Staining reactions : 

 Red blood-corpuscles stain blue. Leukocytes — nuclei 

 stain dark violet ; cytoplasm stains blue. Blood-plate- 

 lets stain light violet. Malarial parasites — nuclei, 

 chromatin portion stains deep violet ; cytoplasm stains 

 light blue. Kresofuchsin, an amorphous powder of 

 gray-blue color, readily soluble in acetic acid and 

 acetone, less readily in alcohol, and only slightly in 

 water ; insoluble in benzene. The alcoholic solution 

 is blue and stains elastin blue ; mucin, cartilage, and 

 homy tissue, reddish ; the aqueous solution is red and 

 stains mucin, cartilage, keratin, and nuclei deep red, 

 but does not stain elastin. Cf. Rothig'' s Stain. 

 Kresylviolet R R, an anilin pigment that may be used 

 as a substitute for the pigments of the methylene-blue 

 group. It has a strong affinity for the chromophilic 

 masses of nerve-cells and gives a metachromic reaction 

 with amyloid substance and with the basophile granules 

 of mast-cells. Cf. the method of Bielschowsky and 

 Plien. Krohnthal's Method for ganglion-cells: Pre- 

 pare lead formate by slowly dropping formic acid into 

 solution of lead acetate ; filter and make an aqueous 

 saturated solution of the white crystals of lead formate; 

 mix with an equal volume of \of c formalin and into 

 this mixture put pieces of brain and spinal cord. After 

 5 days transfer the pieces into a mixture of equal parts 

 of 10 ty formalin and hydrogen sulfid. After 5 days 

 dehydrate in alcohol, embed in celloidin, and mount 

 the sections in xylol-balsam under a cover-glass. Kro- 

 mayer's Method. 1. For fibrin : This is the same 

 as Weigert's method (see Examination of the Blood) 

 except that acetone-xylol (1:5) is used instead of 

 anilin-xylol. 2. For bacteria in sections of epidermis : 

 Apply Weigert's method for fibrin and bleach in 

 acetone-xylol. Krompecher's Method. I. For 

 piasma cells in pathologic tissues : Fix in sublimate or 

 alcohol. Stain the sections for from 15 minutes to 12 



