TEST 



531 



TEST 



scopically, when numerous dark brown crystals of va- 

 rious sizes and forms will be visible. This is not an 

 absolute test, but only corroborative, since substances 

 other than semen give positive results. Formic Al- 

 dehyd. See Denige, Hehner, Hydrochloric Acid, Jo- 

 rissen, Kenimann, Lebbin, Phenylhydrazin, Ramini. 

 Frohn's Reagent. Add 7 gm. of potassium iodid 

 and 10 c.c. of hydrochloric acid to 1. 5 gm. of freshly 

 precipitated bismuth subnitrate which has been treated 

 with 20 c.c. of water and heated to the boiling-point. 

 Fuchsin. Sice Valentine. T., Fuh's, for Albumin. 

 Equal volumes of nonalbuminous urine and a mixture 

 composed of equal parts of carbolic acid and glycerin 

 form an emulsion which clears up on agitation, leaving 

 a transparent and highly refractive liquid. Equal vol- 

 umes of albuminous urine and this solution when 

 mixed produce a white turbidity which remains in 

 spite of agitation and does not precipitate. The test 

 will show o. 1 fy of albumin. Furfurol Reaction 

 for Proteids. Furfurol is produced on hearing pro- 

 teids with sulfuric acid. Fusel Oil. See Saralle. 

 T., Gage's (De M. , for Bacterium coli in water. 

 Dissolve 100 gm. of dextrose and 50 gm. of peptone 

 in a liter of boiling water ; when cool, filter through 

 paper until clear and add 0.25^ of phenol. To loo 

 c.c. of the water to be tested add 10 c.c. of the dex- 

 trose broth and place in an incubator at 38 C. for 20 

 hours, when, if Bacterium coli is present, there will 

 be a bead on the surface. Give the bottle one vigor- 

 ous shake and place it before a window. The gas will 

 separate from liquid and can be seen rising to the sur- 

 face. Gallic Acid. See Bischoff. T., Garxod's, 

 for Hematoporphyrin in Urine. Add to 100 c.c. 

 of urine 20 c.c. of a 10^ solution of caustic soda, and 

 filter. Add to the filtrate thoroughly washed in water 

 absolute alcohol, and enough hydrochloric acid to dis- 

 solve perfectly the precipitate. Spectroscopic exami- 

 nation may now be made of the solution for the two 

 absorption bands characteristic of hematoporphyrin. 

 T., Gautier's, for Carbonic Oxids. Carbon mon- 

 oxid has the power of decomposing iodic anhydrid and 

 forming CO,, the iodin being liberated aud absorbed 

 by copper. It is employed by Niclaux to show normal 

 presence of carbon monoxid in blood. T., v. Geb- 

 hart's, for Glucose. To 10 or 15 drops of urine add 

 10 c.c. of water and a tablet containing sodium car- 

 bonate and orthonitrophenylpropiolicacid; warm care- 

 fully for 2 or 4 minutes, and if sugar is present the 

 mixture becomes greenish and then dark indigo blue. 

 T., v. Gerhardt's. See T, Nitropropiol. Ger- 

 hardt's Reaction for Acetoacetic Acid, G.'s Bor- 

 deaux Reaction. See Test, Gerhardt's, for Diacetic 

 Acid (Illus. Diet.). T., Gerrard's, for Glucose. 

 To a 5^ solution of potassium cyanid add Fehling's 

 solution until the blue Color just commences to disap- 

 pear. On heating this solution to boiling with one 

 containing glucose, no precipitation of cuprous oxid 

 results, but the solution will be more or less decolor- 

 ized. Globulin. See Hammarsten. Glucose. See 

 Baeyer, Eastes, Elliott, Gebhart, Gerrard, Hassall, 

 Kmvarski, Lowenthal, Nickel, Xitrcfropiol, Riegler, 

 Saccharimeter, ll'ender. T., Gluzinske's, for Bile 

 Pigments. Boil the solution for a few minutes 

 with formalin, and an emerald-green coloration 

 will result, changing to an amethyst violet on 

 the addition of a few drops of hydrochloric acid. 

 Glycerin. See Ritsert. T., Grahe's. A test for 

 distinguishing genuine from spurious cinchona barks, 

 made by heating a piece of bark in a test-tube and 

 augmenting the heat to redness. If the bark contains 

 one or more of the cinchona alkaloids, the product 

 formed, condensing as an oily liquid on the sides of 



the tube, will be red. Griess' Red Paper, paper 

 charged with sulfanilic acid and naphthylamin sulfate, 

 used in testing for nitrous acid, nitrites, bilirubin, and 

 aldehyds. Griess' Yellow Paper, paper charged 

 with sulfanilic acid and metadiamido benzene ; used 

 as a sensitive test for nitrites. T., Grigg's, for Pro- 

 teids. A precipitate is formed with all proteids ex- 

 cept peptones on the addition of a solution of meta- 

 phosphoric acid. T., Grinbert's, for Urobilin. 

 Boil together equal parts of urine and hydrochloric 

 acid and shake with ether. In the presence of uro- 

 bilin the ether assumes a brownish-red color with a 

 greenish fluorescence. T., Gunning's, for Acetone. 

 Add to the liquid to be tested an alcoholic solution and 

 then ammonia. When allowed to stand, a precipitate 

 of iodoform and a black precipitate of iodid of nitro- 

 gen are formed ; the latter gradually disappears, when 

 the iodoform is visible. T., Hammarsten's, for 

 Globulin. To the neutral solution add powdered sul- 

 fate of magnesia until no more of the salt dissolves. 

 Separate the globulin thus precipitated by filtration, 

 and wash with a saturated solution of sulfate of mag- 

 nesia. T., HassaU's. The growth of Saccharomy- 

 ces cereznsiie, observed under the microscope, is indica- 

 tive of the presence of sugar in the urine. T., Heh- 

 ner's, for Formic Aldehyd in Milk. Place 5 c.c. of 

 the milk in a test-tube and dilute with an equal volume 

 of water. Carefully pour down the sides of the test-tube 

 strong sulfuric acid containing a trace of ferric chlorid, 

 so as to form a layer of acid below the milk. In 

 the presence of formic aldehyd a violet ring is formed 

 at the junction of the two liquids ; I part in 100,000 

 may be detected. The acid should be of 1.81 to 1.83 

 sp. gr. and must contain a trace of ferric salt. The 

 charring due to the action of the acid on the milk 

 must not be mistaken for color reaction. Helianthin 

 Paper, Methyl Orange Paper, Tropseolin D. 

 Paper, paper charged with methyl orange used in 

 testing for acids and alkalis. Hematoporphyrin. 

 See Garrod. Hemoglobin. See Kobert. T., 

 Hering's. See under Vision, Binocular. T., Hill's. 

 Wrap cotton about one end of a glass rod and so in- 

 sert it in a test-tube that the cotton forms a stopper. 

 Sterilize by dry heat, dip the rod in the material to be 

 tested, return it to the test-tube or put it in a culture- 

 medium. Cf. T, Silk Thread. T., Hofmeister's, 

 for Leucin. A deposit of metallic mercury is formed 

 on warming a solution of leucin with mercurous 

 nitrate. T., Hoppe-Seyler's, for Carbon Mon- 

 oxid in Blood. Add to the blood twice its volume 

 of caustic soda solution of 1. 3 specific gravity. Ordi- 

 nary blood thus treated is a dingy brown mass which, 

 when spread out on porcelain, has a shade of green. 

 Blood containing carbon monoxid, under the same 

 conditions, appears as a red mass which, if spread on 

 porcelain, shows a beautiful red color. T., Hydro- 

 chloric Acid, for formic aldehyd in milk. Heat in 

 a test-tube I c.c. of milk with 4 c.c. of strong hydro- 

 chloric acid containing a trace of ferric chlorid. In 

 the presence of formic aldehyd a purple color appears, 

 varying from a delicate tint to a deep violet. If a 

 yellow color appears, repeat the test, using milk that 

 has been diluted, I to 10, with water. Hydrochloric 

 Acid in Contents of Stomach. See Benedict, 

 Szabo, Villier and Fayolle, Winkler. Hydrocyanic 

 Acid. See Payer. Hydrogen Peroxid. See Bach, 

 ll'urster. Indican. See Oiemuyer. Indigo-car- 

 min Paper, paper charged with indigo carmin 

 and sodium carbonate used as a test for sugar in 

 urine. Indigo Red. See Rosin. Indol. See Baeyer, 

 Xcurki. Iodin. See Sabrazes-Denigk, Sandluna. 

 T., v. Jaksch's, for Uric Acid. Allow the powder 



