PIROPLASMOSIS 43 



3. In the tissues of unfed nymphs bred from infected mothers 

 were round, oval, or pear-shaped bodies (Fig. 10, 8 and 5 1 ), measuring 

 4 fji in diameter, and having peculiar nuclei consisting of one dense mass 

 from which spreads a looser skein of chromatin. These forms were 

 chiefly aggregated in the cells of the salivary glands, suggesting that 

 they were the final stage in the tick, but this proved not to be the 

 case, although they can probably cause infection in the dog, because 

 nymphs in this stage are highly infective. 



4. In squash preparations of unhatched adults 1 bodies occur 

 similar to those last mentioned, but having the nucleus subdivided 

 (Fig. 10 ; 0), aportion of the looser accompanying each subdivision 

 of the denser part of the nucleus, and side by side with such bodies 

 are groups of from three to ten small piroplasmata, especially in the 

 cells destined to form the salivary glands. Christophers thinks 

 that the paired bodies in the gut may represent true conjugation, the 

 large club-shaped bodies may be the ookinets, the bodies in the 

 salivary glands of the nymph be homologous with sporoblasts of the 

 malarial zygote, and the smaller bodies in the adult equivalent to 

 sporozoites. 



1 To form the adults the tissues of the gorged nymphs are, as it were, first 

 melted down and then recast, the salivary glands becoming unrecognisable as 

 such for a time during the process. 



