SMALL-POX AND VACCINIA 



45 



ment ; translated it is as follows : ' If, after forty-eight hours, a slice 

 of a freshly excised inoculated cornea is placed on the warm stage in 

 aqueous humour faintly tinged with methylene blue, the intracellular 

 parasites are distinctly seen to move within their host-cells. When 

 the parasite dies it contracts to a spherical shape, and then becomes 

 of a blue colour. ... At this stage there are no leucocytes at the 

 site of inoculation.' 





^P" Jfcfc.^ 



% 



>v 



FIG. ii. A PORTION OF THE EPITHELIUM OF A RABBIT'S CORNEA FORTY- 

 EIGHT HOURS AFTER VACCINATION. (From a photograph of one of the 

 Author's preparations made in 1894. Stain haema-toxylin and eosin. x 400 

 diameters.) 



Guarnieri's bodies are seen as roundish dark objects near the nuclei of the 



epithelial cells. 



When I demonstrated these bodies before the Pathological 

 Society of London in October, I8Q4, 1 I had vaccinated the cornese 



thus : ' The study of an easily inoculable disease like vaccinia affords material for 

 the comparative study of cancer and sarcoma.' The literature relating to 

 Guarnieri's work and its subsequent developments is so large that even where it has 

 been attempted to give it fully serious omissions have been made. I shall only 

 give references to what appear to me to be the salient contributions to the subject. 

 1 See the Transactions of the Pathological Society, vol. xlvi., p. 192, 1895, with 

 one plate. 



