96 PROTOZOA AND DISEASE 



periphery. This latter change is also shown in Fig. 36 ; 4, where a 

 body with optical characters otherwise exactly resembling the intra- 

 nuclear bodies is seen to be opening out at its surface. Thus, close 

 study of many similar forms proves that the intranuclear bodies 

 usually escape from the nucleus and assume the appearance of 

 amoebae as just described. 



The conclusion may be stated thus : These striking intranuclear 

 bodies do not change into chromatin of the nucleus as part of the 

 mitotic process, but they escape from the nucleus, and, some 

 apparently conjugating together, assume the characters of amoeboid 

 organisms, and subdivide into minute segments, in some cases after 

 having become nucleated. 



The Bodies that contain Chromatin. Apart from the cells that 

 contain resting nuclei, which, except for the large nucleolus-like 

 bodies, are of somatic or of connective-tissue type, such as those 

 provided with a well-marked nuclear membrane e.g., in Fig. 32 ; 

 No. 1 , and eighteen other nucleated cells, and the others that careful 

 examination shows to be resting nuclei of connective-tissue cells 

 without any intranuclear bodies apart from these there are a very 

 great variety of bodies which contain structures that give the re- 

 action of chromatin. Many of them it is impossible to derive from 

 a nucleus of any tissue-cell. It is necessary to examine and draw 

 with care a very great number of these chromatin-containing bodies 

 before a series of average examples can be chosen as the basis of 

 an interpretation of their nature. In tracing such a series the eye 

 is guided by a similarity of optical characters and staining reactions 

 which characterizes these bodies and distinguishes them from the 

 cells of the tissues. In granulation tissue those cells that are under- 

 going mitotic change have a coarser cytoplasmic meshwork and a 

 higher power of refracting light than have the resting connective- 

 tissue cells, but the difference is very slight compared with that seen 

 on comparing the bodies under notice with any form of tissue-cell. 

 Such a series is shown in Fig. 36 ; 5 to 17 inclusive. In some 

 chromatin can be seen streaming from the central part of a body, 

 which, except for the presence of chromatin, resembles that last 



