ON THE INFECTIVE GENITAL TUMOURS OF DOGS 135 



I may now state the chief of these conclusions categorically, 

 referring to the objects depicted in Fig. 53. 



1. Of the intranuclear bodies, some are doubtless the nucleoli of 

 the granulation-tissue cells, but those with definite radiating 

 'tentacles,' (/), are probably parasites which escape into -the cyto- 

 plasm (3 and 4), become nucleated (5 and 6), and there subdivide 

 (7 and 16), either whilst still within the host-cell (7) or after becoming 

 free, as at 27 and 30. 



2. Among the free bodies are some of typically protozoan 

 characters (12, 13, 14 and 15). 



3. Of the cells in mitosis, the bulk of those in the early and 

 medium stages of the tumour (P and 10), are connective-tissue cells, 

 whilst the bulk of those in the fully developed lesion (27, 28, 29 and 

 30, are probably parasites which have become free of the host-cell. 



human sarcoma) ; 2, a cell from a mature lesion containing three nuclei with intra- 

 nuclear bodies ; 3, a similar cell : the stellate body is leaving the nucleus ; 4, a cell 

 in which the nucleus contains no intranuclear body and but little chromatin : a 

 non-nucleated body occupies a cavity in the cytoplasm; 5, a similar cell, with a 

 nucleated amceba-like body in the cytoplasmic cavity , 6, a similar cell, with a 

 rounded nucleated body in the cytoplasm ; 7, a similar cell, with cytoplasmic cell- 

 inclusion in irregular mitosis ; 8, a similar cell, etc., but mitosis is resulting in 

 formation of minute bodies; 9, 10, and 11, three different phases of mitosis, 

 probably tissue-cells; 12, amoeboid parasite with multiple nuclei; 13, 14, and 15, 

 three different amoeboid parasitic cells; 14, showing transition from stellate to 

 amoeboid form ; in i 5 there are two vacuoles ; 16, a cell with empty nucleus and 

 four nucleated cell-inclusions ; 17, part of a capillary containing three leucocytes 

 with typical polymorphous nuclei ; 18, a connective-tissue cell from the part shown 

 in Fig. 51 : the intranuclear body is absent; 19, an adjoining cell in early mitosis : 

 the same as 3 in Fig. 52 ; 20, two cells of the epidermis covering the tumour 

 (Fig. 49), showing perinuclear space and nucleoli : the latter have not the stellate 

 character of those of the tumour-cells ; 21, a capillary of the same tumour with red 

 blood-cells and two leucocytes ; 22^ a group of bodies of different kinds occuring in 

 the central part of the same tumour : the largest cell has a nucleus consisting of a 

 larger cyanophile and a smaller erythrophile part ; 23, mitosis in an epithelial cell 

 in the vaccinated cornea of a rabbit, to show ordinary mitosis in mammalian cell ; 

 24, intracellular nucleated 'vaccine-body' from same preparation as 23; 25, an 

 encapsuled body with nucleus from the middle part of the tumour, as shown in 

 Fig. 49, ;?; 26, an amceboid body entering the cytoplasm of a cell which contains 

 an intranuclear body ; 27, 28, 29, and 30, various forms of mitosis in the central 

 part of tumour (Fig. 49, 3) ; 31, two cells from the sarcoma of breast above show- 

 ing the intranuclear body escaping, below showing gemmule-formation in cyto- 

 plasmic cell-inclusion and empty nucleus. 



In this figure the bodies I, 3, 5, 8, 9, 10, u, 12, 13, 14, and 15 are from the late Dr. 

 Washbourn's section ; the remainder, except where otherwise mentioned, are 

 from Mr. Foulerton's. All are camera drawings; magnification circa 1,000 

 diameters. 



