494 APPENDIX IV. 



way. First place them for a minute or two in alcohol of the 

 strength of that used for staining, and then into absolute alcohol 

 for another like period. Thence transfer to 



(36) Oil of cloves, or, 



(37) Turpentine, and weosote (4:1). 



Sections stained with aniline dyes should be treated as above, but 

 cleared in 



(38) Oil of cedar. 



STAINING. This may be done in a watch-glass, or, better still, in small 

 white earthenware saucers, such as are sold with cheap paint-boxes. 

 especially could those be obtained of larger size, say 1$ or 2- in. in 

 diameter. As a rule, the best staining is obtained by dilute solutions 

 and long treatment The section to be stained must be immersed 

 in the staining fluid, and examined from time to time to judgi- 

 progress. 



The object of staining is to show up (differentiate) diversities of stun 

 ture, either of the plant skeleton or of the cell-contents, each stain 

 particularising some special feature. 

 For a limited list, selected from those in Appendix III., the best HIT : 



(39) Aniline blue. 



(40) Acetic aniline-green. 



(41) Corallin. 



(42) Fuchsin (Magenta) in 50 per cent, alcohol. 



(43) Acetic gentian-violet. 



(44) Methyl-violet in water. 



(45) Safranin in water. 



(These are all aniline dyes, stain rapidly, and the preparations t'air 

 move or U-ss if kept in the light. Safranin is most permanent) 



(46) Mayer's Alum-carmine (Carmalum). 



(47) Grenadier' 1 * logwood (best old). 



In many cases two stains can be used concurrently ; such are: 



(48) Fucli.fi n io'lint'-iirci'ii. 



(49) Eosin-loywood. 



(50) Rosaniline molet. 

 MOUNTING. Preparations can be mounted in 



Glycerine. 

 jelly. 



Hoyer'n mounting fluid (for aniline preparations). 

 Acetate of potash. 



Canada balsam in turpentine or xyloL 

 Dammar. 



No preparations containing water or glycerine can be mounted in 

 Canada balsam or dammar. Watery preparations must be first 

 dehydrated in alcohol ; glycerine preparations first soaked for 

 some time in water, and then in alcohol; and either of them 

 afterwards cleared with oil of cloves. 



