42 



we obain any good results with a modified Eomanowsky dye. 

 Eosin and methylene-blue is the best for exhibiting the plague 

 bacillus, but in using this stain one must be careful in decolorizing, 

 or else too many plague bacilli lose the stain and fallacious con- 

 clusions may be arrived at. This dye, as a rule, fades quickly in 

 the Tropics in an atmosphere saturated with moisture, and hence 

 the sections treated with it must be examined without delay, and 

 in a later reexamination, recoloring frequently becomes necessary.^ 

 The twenty cases to be reported below in detail have been 

 classified imder the following six groups: 



Cases. 



Group I. Primary uncomplicated plague 11 



II. Primary bubonic plague with secondary plague septico- 

 pyemia 4 



III. Primary bubonic plague with secondary plague pneumonia.. 1 



IV. Primary imcomplicated plague pneumonia 2 



V. Primary plague pneumonia with secondary plague septico- 

 pyemia 1 



VI. Primary imcomplicated plague septicaemia 1 



Total 20 



GROUP I. PRIMARY UNCOMPLICATED BUBONIC PLAGUE. 



[Eleven cases.] 



Case Xo. 1. Left Inguinal Bubo. 



[Necropsy Protocol No. 1009. Post-mortem examination July 27, 11 o'clock a. m., 

 about eighteen hours after death, on the body of C. S., a male Chinese, 36 years 

 old, from 217 Santo Cristo. Died July 26 at 4 o'clock p. m.] 



Body of a well-developed, fairly well-nourished, male Chinese 

 about 35 to 40 years old. Post-mortem rigidity not very marked, 

 surface quite cyanotic; post-mortem lividity has made its appear- 

 ance all over the dependent parts and extends in the shape of some 

 irregular patches to the anterior thoracic regions. Some foamy, 

 slightly blood-tinged fluid escapes from the anterior nares. Ko 



^ We believe the reason for the rapid change of the eosin-methylenc-blue 

 to be the following: It is impossible completely to dehydrate sections in 

 an atmosphere saturated with moisture. Hence, after a time the water 

 in the sections will dissolve some eosin, and the eosin solution so formed 

 will in its turn dissolve the methylene-blue. Whether this explanation 

 be correct or not, it does not alter the fact continually observed, that 

 sections stained with eosin and methylene-blue during the rainy season in 

 Manila rapidly fade and, to a great extent, become useless for the study 

 of finer structural details and the presence and distribution of the bacteria. 



