22 BULLETIN 1012, 1*. S. DEPARTMENT OF AGRICULTURE. 



times given in the table are computed from the time when the 

 feedings were finished. 



TABLE S. Time elapsed from feeding to development of symptoms. 



Animal. Time. Animal. Time. 



In some of the animals the symptoms followed immediately after 

 the feeding. The longest time before symptoms was 14 hours and 

 55 minutes in the case of Sheep 449. This probably is longer than 

 the actual time, for the symptoms were first noted in the morning 

 and it is probable that the animal had been sick during the night. 

 Excluding Sheep 449 the average time elapsing between the feeding 

 and the development of symptoms was 2 hours and 5 minutes. On 

 the average the symptoms appeared somewhat more quickly than 

 was the case with Z. paniculatit*. 



RESULT OF AUTOPSYS. 



Opportunity was found for only one autopsy, that on Sheep 599. 

 In this animal the left ventricle was contracted and the right expanded. 

 The left lung was highly congested, and the right lung congested to 

 the point of hepatization. The extreme condition of the right lung 

 was probably due to hypostasis. No other abnormal conditions 

 were noticed. 



TOXIC AND LETHAL DOSAGE. 



Table 6 shows the dosage of the animals poisoned in 1917, 1920, 

 and 1921. 



To determine the probable toxic dosage of the plant it is necessary, 

 of course, to consider only the minimum quantities required to pro- 

 duce intoxication or death. 



It should be noted in connection with this table that although both 

 seeds and fruit heads were used, no results were obtained from the 

 dosage used from these parts of the plant. Sheep 630 received 0.807 

 pound of seed with no effect, and Sheep 594 received 2.437 pounds 

 of fruit heads with no effect. 



So far as Table 6 is concerned, it appears that in general terms the 

 toxic dose of Z. elegans varies from 2 to 4 pounds per 100 pounds of 

 animal. 



Lethal dose. Only one animal, Sheep 599, was killed by Z. elegans. 

 The quantity used in this case was 6.153 pounds of leaves, stems, and 



