38 



BACILLUS RUBER OF KIEL. 



J. BREUNIG. BACTERIOLOGISCHE UNTERSUCHUXG DES TRIXKVVAS- 

 SERS DER STADT KIEL (iNAUG. -DISSERTATION) KIEL, 1888. 



EM. LAURENT. ANNALES DE L'INSTITUT PASTEUR IV, 464, 1890. 



Origin. Drinking waler of Kiel. 



Form. Rods about three to five or seven times as 

 long as wide. 



Motility. Somewhat motile, and the motion de- 

 pends on presence of oxygen. 



Sporulation.--Not observed. 



Anilin Dyes. Stain readily. 



Growth. Rapid and abundant. 



Gelatin Plates. Deep colonies are oval, pale yellow, with wavy or even 

 border. The surface colonies are blood red in color, spread rapidly and have a 

 sinuous border; are surrounded by a clear zone and liquefy gelatin. 



Stich Cultures. Develop along the line of inoculation and liquefaction 

 takes place. The fluid becomes strongly colored and gas may form in the 

 deeper layers. 



Streak Cultures. On agar, at 30-35, the growth is at first a pale rose, and 

 later becomes a brick red. On potatoes, at 30-35, develop rapidly, forming a 

 purple red growth. At lower temperatures the color is less intense, and at 

 first is orange, later carmine red. 



Milk. At 35, coagulation takes place in 24 honrs, without a trace of 

 coloration, due to rapid growth and production of acidity. At ordinary tem- 

 perature the coagulation takes place slowly and the fluid gradually colors. 



Oxygen requirements. Is a facultative anaerobe, 

 but requires oxygen to form the pigment 



Temperature. Grows from 10 to 42C. The opti- 

 mum is 30-35, and above this the growth ceases to be 

 colored. Direct insolation kills it in 5 hours. By exposure 

 of 3 hours is not killed but no longer produces the pig- 

 ment, i. e , becomes attenuated. 



Behavior to Gelatin. Liquefies gelatin quite 

 rapidly. 



Aerogenesis. Some gas bubbles may form in gela- 

 tin tubes. 



Pathogenesis. No action observed. 



