101 



METHODS OF INFECTION. 



1. Cutaneous application. 

 2. Subcutaneous application. 



In mice and rats this can best be done on the back, 



over the root of the tail. 

 3. Subcutaneous injection. 



The Koch syringe is commonly used. Pravaz 



syringe. 

 4. Intravenous injection. 



The large veins in the ears of rabbits are frequently 



used ; also the jugular and femoral veins. 

 5. Intraperitoneal injection. 

 6. Intrapleural injection. 



7. Injection into the anterior chamber of the eye. 

 8. Infection along respiratory tract. 



(a) Inhalation, (b) Injection into the trachea. 

 9. Infection of alimentary canal. 



(a) With food or drink. 



(b) Through a stomach-tube the contents of the 



stomach are previously rendered alkaline. 



(c) Intraduodenal injection. 



The inoculation of animals with pure cultures of 

 microorganisms must be made with rigid precautions to 

 prevent the introduction of foreign organisms. At the 

 site of inoculation the hair must be carefully cut away ; 

 the exposed skin is then well washed with alcohol, and 

 finally is thoroughly moistened with mercuric chloride 

 (1-1000). The instruments employed in making the inoc 

 illations, as knives, scissors, forceps, lance, wire, etc., must 

 be sterilized in a flarne shortly before use. The Koch 

 syringe is sterilized in the dry oven. After having used 

 the instruments they are at once sterilized. 



