Ill 



SPORE STAINS. 



Cover-glass preparations. 

 Air dried. 



Simple: 



12 x through flame. 

 Carbolic-fuchsine (hot 



inin.). 



Water (and examine). 

 Air dried. 

 Canada balsam. 



Double: 



3 x through flame. 

 Carbolic-fuchsine (hot 2 to 



5 min ). 



Water (and examine). 

 Dilute alcohol. 

 Water (and examine). 

 Contrast color (metbylene 



blue, ]- to min.j. 

 Water (and examine). 

 Air- dried. 

 Canada balsam. 



2; to Metchnikoff's cellular 

 theory of immunity, the white blood cell is endowed with 

 the power of taking into itself, and ultimately destroying, 

 the invading organism. Phagocytic action can be readily 

 demonstrated in frog-* inoculated with anthrax. For this 

 purpose a pure culture of the amiirax baciilus is intro-. 

 duced into the dorsal lymph sac of a frog, and at the end 

 of 12 or 18 hours it is killed with chloroform. 



Make cover glass preparations with the. fluid in the 

 dorsal lymph sac and stain some with simple anilin dyes 

 and others after Gram's method. 



Pliagocytes. Accordin; 



i i 



