125 



Ion, agar, etc., are inoculated in the usual manner. The 

 projecting part of the cotton plug is cut off close to the 

 mouth of the tube, and the plug slightly raised, with 

 sterilized forceps, to facilitate diffusion of the gas. The 

 tube is then placed in the bottle by means of a pair of 

 long forceps and the apparatus connected with a Kipp's 

 hydrogen generator. The current of hydrogen should be 

 passed first through an alkaline solution of lead acetate, 

 and then through a six per cent, solution of potassium 

 permanganate. After passing through the apparatus the 

 gas passes through a small wash-bottle containing water, 

 which serves as a valve. If carbonic acid is used it should 

 be passed through a saturated solution of sodium carbonate. 

 A rapid current of gas is passed through the bottle for 1 to 

 2 hours, it is then sealed by turning the stopper, and set 

 aside in the incubator to develop. 



The apparatus can also be used for vacuum cultures, 

 in which case it is connected with a Chapman aspirator 

 and the air pumped out. The alkaline pyrogallate method 

 can be employed with excellent results. (NovY, Central- 

 Uatt fur Bakteriologie, 14, 581, 1893). 



By far the easiest and simplest method for obtaining 

 plate cultures of anaerobic bacteria is that devised also in 

 this laboratary. The apparatus, which has the form of a 

 desiccator, is provided with the special stopper seen in 

 the bottle, Fig. 1. Petri plates are placed in the appara- 

 tus, hydrogen is then passed through for 1 to 2 hours, 

 and finally it is sealed by turning the stopper. 



LABORATORY WORK. Liquefy four glucose agar tubes 

 by heating in the water bath ; then allow to solidify in an 

 upright position. When cool make deep stich cultures of 



Bacillus of symptomatic anthrax. 

 Bacillus of malignant oedema. 

 Bacillus of malignant oadema, No. II. 

 Bacillus of tetanus. 



