128 



Summary for staining flagella : 



Dilution cover-glass preparation. 

 Air-dried. 

 1 x through flame. 

 Mordant, hot (1 to 2 min.). 

 Water. 



Alcohol (few seconds). 

 Anilin-water fuchsine, hot (1 to 2 min.). 

 Water (and examine). 

 Air-dried. 

 Canada balsam. 



The mordant employed is prepared by dissolving 20 g. 

 of tannic acid in 80 c. c. of distilled water. To 10 c. c. of 

 this tannic acid solution, add 5 c. c. of ferrous sulphate 

 solution (1-2), and 1 c. c. of saturated alcoholic solution of 

 fuchsine. 



The stain employed is made by adding 4 to 5 g. of 

 fuchsine to 100 c. c. of anilin water (p. 106). Both mor- 

 dant and stain should be kept warm while in use. 



