152 



VIBRIO OF DENEKE. (1885). 



SPIRILLUM TYKOGENUM. SPIRILLUM OF DENEKE. CHEESE SPIRILLUM. 



Origin. From old cheese. 



Form Sliirhtly bent rods, which forms spirals, and 

 resemb'e greatly the cholera vibrio. 



Motility. Very motile. 



Speculation. Not known. 



Anilin Dyes. Stain readily. 



Growth. Is quite rapid, but less than (hat of the 

 Finkler-Prior vibrio. On some gelatin media it liquefies 

 very slowly or not at all. 



Plates. The colonies which develop on c/clatin plates are yellowish, 

 liquefy and the plate as a whole may resemble somewhat that of the cholera 

 vibrio, but the liquefaction is more rapid. Under the microscope they are 

 seen to be irregular coarsely granular, and the center is yellowish-green. 



8ticJi Cultures. Growth and liquefaction takes place in gelatin tubes 

 along the entire line of inoculation. The mass of bacteria settle to the bottom 

 while on the surface a yellowish scum or layer forms. 



Streak Cultures. On agar a thin yellowish growth develops along the 

 line of inoculation. On potatoes, in the incubator, it forms a delicate, yel- 

 lowish covering which frequently contains well-formed spirals. On blood 

 serum, cultures soon produce liquefaction. 



Oxygen requirements. Is a facultative anaerobe. 



Temperature. Grows at ordinary temperature, also 

 at 37 0. 



Behavior to Gelatin. Liquefies more rapidly than 

 the cholera vibrio. 



Aerogenesis. Not observed. 



Pathogenesis Is less pathogenic to guinea pigs 

 lhan the Finkler Prior vibrio. 



