193 



in this slain for 15 to 30 minutes. They are then washed 

 in anilin water to remove excess of the dye, and thus to 

 prevent the formation of unsightly deposits on subsequent 

 contact with iodine. The sections are then placed in the 

 solution of iodine in potassium iodide (p. 107) for 2 or 3 

 minutes. From this they are transferred to absolute alco- 

 hol and gently moved about till most of the stain is re- 

 moved. The sections should be still slightly stained, not 

 completely decolored. They are then placed in Weigert's 

 picrocarmine solution or in eosine, for 1-2-3 minutes, de- 

 hydrated in absolute alcohol for a few seconds and then 

 transferred to oil of cloves in which the sections are al- 

 lowed to remain till all the gentian violet lias been re- 

 moved. Oil i/f cloves, especially when dark colored, has 

 strong decolorizing properties and will remove all traces 

 of gentian violet from the tissues without affecting the 

 bacilli to any extent. The sections are then placed jn 

 xylol and examined and if satisfactory mounted in Can- 

 ada balsam. 



ANTHRAX SECTIONS. 



Simple Stain: Gra'tns Method: 



Dilute anilin stain Anilin water-gentian violet 



(10 to 30 min.). (warirr 15 to 30 min.). 



Acetic water. Iodine in potassium iodide 



Water (and exam- (2-3 min.). 



ine). Absolute alcohol. 



Absolute alcohol Picrocarmine (1-2-3 min.). 



(few seconds). Absolute alcohol (few 



Oil of cloves. seconds). 



Xylol (and exam- Oil of cloves (till violet 



ine). ceases to be given off). 



Canada Balsam. Xylol (and examine). 



Canada balsam. 



Malignant oedema. Prepare sections from the ab- 

 dominal wall, kidney and liver of a guinea-pig which died 

 after inoculation with the bacillus of malignant oedema. 



