194 



The tissues and organs should be removed 48 hours after 

 the death. Stain with dilute carbolic fuchsine according 

 to Pfeiffer's method as given on page 192. 



Symptomatic anthrax. Prepare sections from the 

 same tissues as above from a guinea-pig which was not ex- 

 amined till 48 hours after the death. Stain by the same 

 method and compare the two organisms. 



Bacillus cedematis maligni, No. II. Section the 

 thickened abdominal wall and stain after Gram's method. 



Tubercle bacillus. Prepare sections of tubercular 

 human lung, also of the spleen, liver, and mesenteric tu- 

 bercles of a guinea-pig inoculated with tubercular spu- 

 tum. Stain the sections according to the following method 

 which is a modification of the Ziehl-Neelsen method. 



The sections are placed in Ziehl's carbolic fuchsine, 

 slightly warmed, for 15-30 minutes. They are then trans- 

 ferred to Ebner's solution where they are moved about 

 till the color ceases to be given off. The sections should still 

 possess a slight pink color. They are then placed in dilute 

 methylene blue for ^-1 minute. From this they are trans- 

 ferred by means of a spatula to absolute alcohol for -J-l 

 minute. The sections must not remain in the alcohol till 

 all the blue disappears. They are then placed in oil of 

 anise, transferred to xylol and examined. If satisfactory 

 the section is mounfed in Canada balsam. 



Ebner's decalcifying solution is prepared according to 

 the formula: Sodium chloride 0.5, hydrochloric acid 0.5, 

 alcohol 100, distilled water 30. 



Instead of using Ebner's solution for decoloring the tis- 

 sues a 2 per cent, aqueous solution of anilin hydrochloride 

 can be employed with excellent results as it has little or 

 no tendency to decolor the tubercle bacilli. (KiiHNE, 

 BORREL). The sections are stained in Ziehl's solution as 

 above, then placed for a few seconds in the 2 per cent, 

 aqueous solution of anilin hydrochloride, then washed in 

 alcohol and counter-stained as above. 



Leprosy bacillus. Sections of the skin of a leper 



