195 



can be double stained according to the method described 

 for the tubercle bacillus. It can be summarized as fol- 

 lows : 



Sections. 



Carbolic fuchsine (warm 15-30 min.). 



Ebner's solution (till it turns a light pink). 



Dilute methylene blue (-J 1 min.), 



Absolute alcohol (-J-1 min.). 



Oil of anise. 



Xylol (and examine). 



Canada balsam. 



Leprosy sections left in dilute alcohol soon lose their 

 capacity for double staining. They can be simple stained 

 with methylene blue, or with carbolic fuchsine by Pfeif- 

 fer's method. 



Glanders bacillus. The detection of this bacillus 

 in tissue i? rather difficult owing to its marked peculiarity 

 of readily becoming decolorized. Sactions from the 

 spleen, of a guinea pig should be simple stained with 

 Lo filer's alkaline meihylene blue (p. 140) or with carbolic 

 fuchsine. 



Typhoid fever bacillus. The E berth bacillus al- 

 though it is siained readily and intensely is very likely 

 to become decolorized in the ordinary method of staining. 

 Sections of human spleen are stained in Loffler's alkaline 

 methylene blue for 24 hours. Then washed and decolored 

 in water, dehydrated in anilin oil, allowed to dry on a slide 

 and finally cleared up with xylol. Simple stains can be 

 made with carbolic fuchsine, decolorizing carefully in acid- 

 water and alcohol. 



Frankel's diplococcus. Sections from the lung, 

 spleen, liver etc., of a rabbit can be stained by Gram's 

 method, 



Loffler's diphtheria bacillus. Sections of diph 

 thentic membranes, or of muscles from the neighborhood 

 of the point of inoculation should be stained with Loffler's 

 alkaline methylene blue, or by Gram's method. 



