76 MASTITIS OF THE COW 



bouillon culture of bacillus pyogenes. The streptococcus 

 and also the bacillus pyogenes were isolated from a case 

 of mastitis. The streptococcus killed two control mice, 

 the first in one and the second in two days. The virulence 

 of the bacillus pyogenes was not controlled. 



Examination of the infected quarter and its milk before 

 infection revealed no pathological alterations. 



On the day after infection the body temperature was 

 40.5° C. (ca. 105° F.). The quarter was severely swollen 

 and painful, the quantity of milk was decreased and the 

 milk was yellowish-white and thick and showed sediment 

 consisting of pus which contained a great number of strep- 

 tococci but no pyogenes bacilli. 



Two days after the infection the body temperature was 

 39.8° C. (ca. 103.5° F.). The milk was yellow and puru- 

 lent and contained shreds of croupous membrane showing 

 a few streptococci. It was otherwise as before. 



Three days after the infection the body temperature 

 was 39.3° C. (102.5° F.). It was otherwise the same as 

 before. 



Four days after the infection the body temperature 

 was normal. The swelling of the quarter was somewhat 

 reduced. It was otherwise the same as before. 



During the following day this condition remained the 

 same. 



Six days after the infection the quarter showed firm 

 nodular swellings here and there. The milk was yellowish- 

 gray, purulent and contained croupous membranes which 

 showed a few streptococci and here and there in the cells 

 small groups of pyogenes bacilli. 



Dm*ing the following days this condition remained 

 unchanged. 



Twenty days after the infection the quarter was in the 

 same condition. The milk was yellow and pus-like and con- 

 tained free croupous membranes showing few streptococci 



