5 



Bacteria are, added, but when sensitized bacteria are added to such a 

 suspension of leucocytes there is good phagocytosis. This work was 

 confirmed and extended by Hektoen & Ruediger** and by Bulloch & 

 Atkin.*** In consideration of these facts we would expect that the 



TABLE 2. 



-SHOWING THE EFFECT OF GUINEA-PIG*S I.EUCOCYTIC EXUDATE AND MIXTURE OF EXU- 

 DATi: AND BODY FLUIDS' ON STREPTOCOCCI. 



Strepto- Immedi- 



cocci. ately. 3 hrs. 18 hrs. 



300 Leucocytic exndate 6500 70 170 



A Leucocytic exndate 650 600 Many 



B104 Leucocytic exudate 8000 10000 Many 



300 Cell-free exudate 5500 800 GOflO 



7H04 Cell-free exudate 8000 10000 Many 



300 Defibrinated blood .6000 5000 Many 



300 Defibrinated blood + leucocytes 6800 240 72 



300 Serum 6000 6000 Many 



300 Serum + leucocytes 6000 2000 Many 



Streptococcus Xo. 300 is not virulent for guinea-pigs. Nos. B104 and A are 

 virulent. No. A was isolated from a peritonsillar abscess following an attack 

 of follicular tonsillitis and was used in this experiment in the first generation 

 on artificial media. 



TABLE 3. 



PHAGOCYTOSIS OF STREPTOCOCCI BY RABBIT LEUCOCYTES AND GUINEA-PIG LEUCOCYTES. 



Strepto- Phagocy- 



cocci. tosis. 



300 Rabbit leucocytes in blood 20 



B104 Rabbit leucocyte? in blood : . 



300 Guinea-pig leucocytes in blood 30 



13104 Guinea-pig leucocytes in blood 1 



300 Washed rabbit leucocytes in NaCl solution 1 



300 Washed rabbit leucocytes in heated rabbit serum 1.5 



300 Washed rabbit leucocytes -f sensitized streptococci 12 



Each tube contained 2 c. c. of suspension of leucocytes -(- 2 c. c. of suspen- 

 sion of streptococci. Tubes were incubated 1 hour, smears were made and 

 stained and the degree of phagocytosis determined by counts. 



extent of destruction of streptococci in the test tubes should run parallel 

 with the degree of phagocytosis of these organisms and Table 3 shows 

 that this is the case. * ' 



If we read the three tables together we notice that the virulent 



**Jour. of Infect. Dis., 1905, II, 128. 

 ***Proc. of. Royal Soc., 1905, LXXIV, 379. 



