KILUNli. 15 



i (Minna n. Flora Golf. Neapel, 16, 1887, p. 239) 

 benumbs Capitellida) by putting' them into a mixture of one 

 part of 70 per cent, alcohol with 9 parts of sea water. 



OESTEEGREN (Zeit. wi$s. Mik., xix, 1903, p. 300) makes a 

 saturated (7 to 8 per cent.) solution of ether in sea or soft 

 water, and uses it either concentrated or diluted to about 1 

 per cent., and finds it to succeed with all classes of aquatic 

 animals. 



COKI (Zeit. wisv. Mik., vi, 1890, p. 438) recommends a 

 mixture composed of 10 c.c. in ethy 'I- alcohol (of 96 per cent, 

 strength), 90 c.c. water (fresh or sea water), and 0'6 grm. of 

 sodium chloride (to be added only when fresh water is 

 taken, the addition of the salt having for its object to 

 prevent maceration). It may be well to add to this mixture 

 a very few drops of chloroform (for Cristatella Ze.it. wiss. 

 ZooL, lv, 1893, p. 626). 



19. Chloreton (Aceton Chloroform) is recommended for inver- 

 tebrates and larvas of Rana by RANDOLPH (ZooL Anz. y xxiii, 

 1900, p. 436). KRECKER (Zeit. wiss. ZooL, xcv, 1910, p. 

 383) takes solutions of -J- to 1 per cent, for Oligochaeta. 

 SULIMA (Zeit. Biol. Techn., Strasburg, i, 1909, p. 379) takes 

 a mixture of 99 parts of sea water and 1 of 10 per cent. sol. 

 of chloreton in absolute alcohol, for Scy Ilium and Anguilla. 



20. Hydrate of Chloral. FOETTINGER (Arch, de Biol., vi, 

 1885, p. 115) operates by dropping crystals of chloral into 

 the water containing the animals. For Alcyonella he takes 

 25 to 80 centigrammes of chloral for each hundred grammes 

 of water. It takes about three-quarters of an hour to 

 render a colony sufficiently insensible. He has obtained 

 satisfactory results with marine and fresh-water Bryozoa, 

 with Annelida, Mollusca, Nemertians, Actiniae, and with 

 Aster acantUion. He did not succeed with Hydroids. 



Lo BIANCO (Mitth. ZooL Stat. Neapel, Bd. ix, 1890, p. 

 442) employs for various marine animals freshly prepared 

 solutions of chloral in sea water, of from one tenth to one 

 fifth per cent, strength. 



I have never had the slightest success with Nemertians. 



YERWORN (Zeit. iviss. ZooL, xlvi, 1887, p. 99) puts Cristatella for a 



