48 CHAPTER V. 



The addition of sodium chloride allows a stronger solution to be 

 obtained than can be made with pure water, and also, it is stated, 

 enhances the penetration of the sublimate. But the fixation-precipitates 

 ( 29) formed by the double salt are (according to SPULER, Encycl. inik. 

 Technih., p. 1274) for the most part soluble in water, thus giving rise to 

 imperfect preservation. 



Concentrated (i. e. over 20 per cent.) solution in sea-water is recom- 

 mended for some marine animals. 



STOELZNER (Zeit. wiss. MiJcr., xxiii, 1906, p. 25) recommends saturated 

 solution of sublimate in sugar solution of 4 per cent., as isotonic (for 

 warm-blooded animals). 



Liquid of Lang (Zool. Anzeiger, 1878, i, p. 14). For Planaria. 

 Distilled water .... 100. 

 Chloride of sodium . . . 6 to 10. 

 Acetic acid . . . . 6 to 8. 

 Bichloride of mercury . . 3 to 12. 

 (Alum, in some cases . . ^.) 



65. Alcoholic Solutions. APATHY (Mikrotechnik, p. Ill) 

 recommends a solution of 3 to 4 grammes of sublimate 

 and 0*5 gramme sodium chloride in 100 c.c. of 50 pel- 

 cent, alcohol, for general purposes. 



OHLMACHER (Journ. Exper. Medicine, ii, 6, 1897, p. 671) takes- 

 Absolute alcohol 80 parts. 



Chloroform 15 ,, 



Glacial acetic acid 5 



Sublimate to saturation (about 20 per cent.). 



" Ordinary pieces " of tissue are sufficiently fixed in fifteen to thirty 

 minutes. Entire human cerebral hemispheres, subdivided by Meynert's 

 section, take eighteen to twenty-four hours. 



For liquids containing a much higher proportion of acetic acid, see 

 Acetic Alcohol. 



66. Aceton Solution. HELD (Arch. Anat. Phys., Anat. Abth., 

 1897, p. 227), fixes nerve-tissue in a 1 per cent, solution of 

 sublimate in 40 per cent, aceton, and washes out through 

 increasingly concentrated grades of aceton. 



67. Phenol Solution. PAPPENHEIM (Arch. Path. Anat., civil, 1899, 

 p. 23) shakes up carbolic acid with aqueous sublimate solution and 

 filters. 



68. Ciaccio (Arch. Hal. Anat. Embr., vi, 1907, p. 486) has an irrational 

 mixture of sublimate, iodine, and formol. 



69. Mercuro-nitric Mixtures. FRENZEL (Arch. mik. Anat., 

 xxvi, 1885, p. 232) recommends a half-saturated solution of 



