50 ('IIAI'TKK V. 



vol. ; a similar solution of picric acid, 1 vol. ; distilled 

 water 2 vols. Embryos may be left in it for twelve hours 

 washed for two hours in water, and brought into weak 

 alcohol. 



MANN'S (op. cit., xi, 1895, p. 480). 1 per cent, of picric acid with or 

 without 1 per cent, of tannin in a saturated solution of sublimate in 

 normal salt solution. 



The same author's Alcoholic Picro-sublimate (Anat. ^4/iz.,viii, 1893, 

 pp. 441 4io) consists of absolute alcohol 100 c.c., picric acid 4 grins. , 

 sublimate 15 grnis., tannin 6 to 8 grins. The tannin is added in order 

 to prevent excessive hardening. 



PACAUT (Arch. Auat. Micr., viii, 1908, p. 438), takes 200 parts of 

 saturated solution of sublimate and picric acid, 6 of 3 per cent, platinum 

 chloride, and 5 of 16^ per cent, solution of chromic acid. 



0. vom RATH (Anat. Anz., xi, 1895, p. 268) takes cold 

 saturated solution of picric acid, 1 part ; hot saturated 

 solution of sublimate, 1 part ; glacial acetic acid, ^ to 1 per 

 cent. Also the same with the addition of 10 per cent, of 2 

 per cent, osmic acid solution. 



FISH (Trans. Ainer. Micr. Soc., xvii, 1896, p. 143) takes 1 litre of 

 water, 1 g. picric acid, 5 g. sublimate, and 10 g. acetic acid. 



LENHOSSEK (Intern. Monatschr. Anat. Phys., xxiv, 1907, p. 293) takes 

 saturated sublimate 75 c.c., acetic acid 5, 50 per cent, alcohol 25, and 

 picric acid to saturation. 



71. Osmio-sublimate Mixtures. MANN'S (Zeit. u-iss. Mik., xi, 

 1894, p. 481) consists of a freshly prepared mixture of equal 

 parts of 1 per cent, osmic acid solution and saturated 

 solution of sublimate in normal salt solution (for nerve- 

 centres). 



DBUNER'S (Jena. Zeit. Naturw., xxviii, 1894, p. 294) con- 

 si.-ts of 1 part of 1 per cent, osmic acid solution added to 20 

 parts of a solution of 5 per cent, each of sublimate and 

 glacial acetic acid in water. 



O. vom RATE'S, see last . 



72. Chromo- sublimate. Lo BIANCO (Mitth. Zool. Stat. 

 Neapelj ix, 3, 1890, p. 443). Concentrated sublimate solu- 

 tion, 100 parts, 1 per cent, chromic acid, 50 parts. 



MANN (Verh. Anat. Ges., 12, 1898, p. 39) takes for nerve-cells equal 

 parts of 5 per cent, sublimate and 5 per cent, chromic acid. 



73. Sublimate and Bichromate. ZI^KEK'S Mixture (Munchener 



