FIXING AND HARDENING AGENTS. 51 



med. Wochenschr., 24, 1894, p. 534 ; quoted from MERCIEK, 

 Zeit. wiss. Mik., xi, 4, 1894, p. 471). Five per ceufc. of 

 sublimate and 5 per cent, of glacial acetic acid dissolved in 

 solution of MULLER. Fix for several hours, wash out with 

 water, treat the tissues in bulk, or the sections with alcohol 

 containing tincture of iodine. 



See also RETTERER, Joum. Anat. Phi/s., xxxiii, 1897, p. 

 463, and xxxvii, 1901, p. 480. 



If the objects be allowed to remain too long in the fluid 

 there may be formed precipitates, which it is very difficult to 

 remove. SPULER (Encyd. mik. Techmk.,lat edition, p. 1280) 

 says that they may be avoided by removing the objects as 

 soon as penetrated, and completing the hardening in liquid 

 of MULLER. 



DAHLGREN'S modification, consisting of equal parts of 

 M tiller's solution and saturated sublimate solution with 5 per 

 cent, of glacial acetic acid, gives fewer precipitates (SPULER, 

 I. c.). 



HELLY (Zeit. wiss. Mik., xx, 1904, p. 413) omits the acetic 

 acid, and adds, immediately before use, 5 per cent, of formol. 



MAXIMOW (ib., xxvi, 1909, p. 179) adds 10 per cent, of 

 formol and sometimes 10 per cent, of osmic acid of 2 per cent, 

 (fix in the dark). 



FOA (Quart. Journ. Mic. 8cL, 1895, p. 287) takes equal parts of 

 saturated solution of sublimate in normal salt solution, and of liquid of 

 Miiller, or 5 per cent, solution of bichromate. 



BENSLEY (Proc.. Canadian Inst., v, 1897, p. 77 ; Zeit. wiss. Mik., xvii, 

 1900, p. 233) takes equal parts of saturated solution of sublimate in 96 

 per cent, alcohol and 2 per cent, solution of bichromate in water. 

 Wash out in 50 per cent, alcohol. 



HOYER (Arch. MiTcr. Anat., liv, 1899, p. 97) takes 1 part 5 per cent, 

 sublimate and 2 of 3 per cent, bichromate. 



KOHN (ib., Ixx., 1907, p. 273) takes 5 parts 5 per cent, sublimate, 15 

 parts 3 1 per cent, bichromate, and 1 part acetic acid. 



ARNOLD (Arch. Zellforsch., iii, 1909, p. 433) takes 2*5 parts bichromate, 

 1 of cupric sulphate, 10 of acetic acid, and 100 of saturated solution of 

 sublimate. 



74. Sublatnin (Ethylendiamin Sulphate of Mercury) is recom- 

 mended in 5 per cent, solution by KLINGMULLER and YEIEL, Zeit. wiss.. 

 MiJcr., xxi, 1904, p. 58. 



75. Platinum Chloride. The substance used and intended by 

 the authors who have recommended this reagent is not the 



