66 CHAPTER 



Mic. Soc., 1898, p. 4!>2) are in my view too weak, and suffer by the 

 omission of the acetic acid. 



MOREAUX (Bibl. And., 1910, p. 265) takes 15 parts formol, 85 of 

 triclilor-acetic acid of 3 per cent., and picric acid to saturation. 



111. Picro-platinic Formol (M. and P. BOUIN, Bibl. Aitat. } 

 1898, f. 2, p. 2).- 



Platinuni chloride, 1 per cent. sol. . 20 parts. 

 Picric acid, saturated sol. . . 20 



Formol . . . . . 10 



Formic or acetic acid . . 5 



I find this excellent, but the mixture does not keep nioro 

 than a day or two. 



BOUIN also (Arch. Biol., xvii, 1900, p. 211) simply 

 substitutes formol for the osmic acid in HERMANN'S mixture 1 ,, 

 45. 



112. Sublimate Formol (M. and P. BOUIN, loc. cit.).A similar 

 mixture, in which sublimate of 1 per cent, is substituted for the plati- 

 num chloride. 



Another formula of the same authors (Arch. Biol., xvii, 1900, p. 211) 

 is 1 part of formol to 3 of saturated aqueous sublimate. Rinse with 

 water and bring into alcohol of 70 per cent. 



SPULER (E)icycl. mik. Technik., 1st ed., p. 1280) adds to sublimate of 3 

 per cent, or more 1 per cent, of glacial acetic acid and 10 per cent, of 

 formol. 



MANN (Verh. Anat. Ges., 1898, p. 39) takes for nerve-cells 2^ g. sub- 

 limate, 1 g. picric acid, 5 c.c. formol, and 100 c.c. water, or (Methods, 

 etc., p. 97) for all tissues 2 g. sublimate, 20 c.c. formol, and 80 c.c. water. 



BRANCA (Journ. Anat. et Phys., xxxv, 1899, p. 767) adds 10 parts of 

 formol and 1 of acetic acid to 60 parts of saturated solution of picric 

 acid in saturated aqueous sublimate. 



NOWAK (Anat. Anz., xx, 1901, p. 244) takes 30 parts of saturated sub- 

 limate, 30 of 1 per cent, chromic acid, 27 of water, 3 of acetic acid, and 

 10 of formalin. 



113. Formol-Muller. This is the name given by Oirni 

 (Berl. Idin. Wochenschr., 1896, No. 13) to a mixture of 1 

 part of formol with 10 of liquid of Miiller ( 53). It should 

 be freshly made up. Fix for three hours in the stove, or 

 twelve at normal temperature, wash out with running water. 

 Much used, especially for nervous tissues. 



MOELLER (Zeit. wiss. Zool. y Ixvi, 1899, p. 85) takes 1 vol. 

 of formol and 4 of 3 per cent, bichromate (for the intestine 

 of mammals). 



