114 CHAPTER IX. 



STEPANOW (Zeit. wiss. Mik., xvii, 1900, p. 185) soaks and imbeds in a 

 solution of celloidin in a mixture of equal parts of ether and clove oil, 

 hardens in alcohol or vapour of chloroform, or in benzol, and cuts either 

 wet or dry. 



See also TSCHERNISCHEFF, ibid, p. 449. 



JORDAN, ibid., p. 193, imbeds in a mixture of 5 parts of 8 per cent, 

 celloidine solution with 1 of oil of cedar, hardens first in vapour of 

 chloroform and then in a mixture of 5 parts of chloroform with 1 of oil 

 of cedar, and cuts wet or dry. 



171. Double Imbedding in Collodion and Paraffin. This is 

 sometimes, though rarely, employed for objects of which it is desired to 

 have very thin sections, and which are too brittle to give good sections 

 by the plain paraffin process. 



KULTSCHITZKY'S Method (Zeit. wiss. Mik., iv, 1887, p. 48). After 

 the collodion bath, the object is soaked in oil of origanum (Oleum 

 Origani vulg.). It is then brought into a mixture of origanum oil and 

 paraffin heated to not more than 40 C., and lastly into a bath of pure 

 paraffin. 



The mass may be preserved in the dry state, and may be cut dry. 

 RYDER (Queen's Micr. Bull, 1887, p. 43 ; Journ. Roy. Micr. Soc., 1888, 

 p. 512) modified the process by substituting chloroform for the 

 origanum oil. 



IDE (La Cellule, vii, 1891, p. 347, and viii, 1, 1892, p. 114) imbeds in 

 collodion in a tube by GILSON'S process ( 169) ; the collodion is boiled 

 for forty minutes, then brought for fifteen minutes (this is for small 

 objects) into chloroform heated to 30 C. containing one fourth part of 

 paraffin dissolved in it, then for ten minutes into pure melted paraffin. 



FIELD and MARTIN (Bull. Soc. Zool. de France, 1894, p. 48) make a 

 solution of dried celloidin in a mixture of equal parts of absolute alcohol 

 and toluene, of about the consistency of clove oil. This solution is 

 saturated with paraffin, added in shavings at a temperature not exceed- 

 ing 20 to 23 C. The tissues are prepared by soaking in some of the 

 mixture of alcohol and toluene, and are then penetrated with the 

 celloidin-paraffin solution. The mass is hardened in a saturated solution 

 of paraffin in chloroform or in toluene, and is finally imbedded in pure 

 paraffin in the usual way. 



STEPANOW imbeds in paraffin after clearing with benzol, last . 

 JORDAN, after imbedding as in last , passes through a bath of 

 j :i raffin dissolved in chloroform into pure paraffin. 



WILHELMI (Fauna Flora Golf. Neapel, xxxii, 1909, p. 17), following 

 APATHY, imbeds in celloidin, hardens in chloroform, then adds benzol 

 to the chloroform, and passes through pure l^enzol (half an hour to an 

 hour) into paraffin, and cuts dry. 



Similarly, BRECKNER, Zeit. wiss. Mik., xxv, 1908, p. 29. 

 STERLING (Jena Zeit., 1909, p. 253) soaks for two or three days in 

 equal parts of clove oil and collodion, puts for a couple of hours (until 

 clear) into xylol, and imbeds in paraffin. 

 See also DAHLOREN, Journ. Appl. Microsc., 1898, p. 97 ; SABUSSOW, 



