CHAPTER X. 



SERIAL SECTION MOUNTING. 



185. Choice of a Method. I recommend the following : 

 For general work with paraffin sections, the combined water 

 and albumen method, 188. For very delicate work, the 

 water method. For collodion sections, the albumen method ; 

 for large collodion sections, GRAHAM KERB'S seems the most 

 convenient. 



Methods for Paraffin Sections. 



186. The Water or Dessication Method. GAULE (Arch. Anat. 

 Phys.j Phys. Abth., 1881, p. 156) ; SUCHANNEK (Zeit. wiss. Mik., 

 vii, 1891, p. 464) ; GDLLAND (Journ. Anat. and Phys., xxvi, 

 1891, p. 56) .; SCHIEFFERDECKER (Zeit. iciss. Mik., ix, 1892, p. 

 202) ; HEIDENHAIN (Kern und Protoplasma, p. 114) ; NUSBAUM 

 (Anat. Anz., xii, 2, 1896, p. 52) ; MAYER in the Grundzuge, 

 LEE und MAYER, 1898, p. 113; DE GROOT (Zeit. wiss. Mik., 

 xv, 1898, p. 62), and others. The principle of this method 

 is that the sections are made to adhere to the slide without 

 the intervention of any cementing substance, being brought 

 into intimate contact with the glass by being slowly drawn 

 down by the evaporation of a layer of water on which they 

 are floated. It is now practised, with unessential variations, 

 as follows : 



(a) For sections that are large and not numerous. The 

 sections are flattened out on water by one or other of the 

 processes described in 149. The slide is then drained and 

 put away to dry until every trace of water has completely 

 evaporated away from under the sections. This drying may 

 be performed at the temperature of the laboratory, in which 

 case many hours will be necessary (to be safe it will generally 

 be necessary to leave the sections overnight). Or it maybe 



