1 () 1 ril.APTKK XIII. 



etc., 1892) recommends, under the name of " acid haematoxylin," solution 

 of Delafield very strongly diluted, and with enough acetic acid added to 

 it to give it a decidedly red tint. This gives a sharper and more differ- 

 entiated nuclear stain than the usual solution. 



MARTINOTTI (Zeit. wiss. M>lc., xxvii, 1910, p. 31) makes it up with 0'2 

 per cent, of hsematein, and less alum (2 per cent.). 



256. Ehrlich's Acid Haematoxylin (Zeit. ?n>*. Mil'., 1886, p. 

 150). Water 100 c.c., absolute alcohol 100, glycerin 100, 

 glacial acetic acid 10, haematoxylin 2 grms., alum in excess. 



Let the mixture ripen in the light (with occasional 

 admission of air) until it acquires a dark red colour. It 

 will then keep, with constant staining power, for years, if 

 kept in a well-stoppered bottle. It is very appropriate for 

 staining in bulk, as over-staining does not occur. I find it 

 excellent. 



MANN (ibid., xi, 1895, p. 487) makes up this stain with an 

 equal quantity of haematein instead of haematoxylin. 



MATE it (GrundzJiye. LEE and MAYER, first edition, p. 154) 

 finds that this is too much and makes the mixture overstain ; 

 04 grin, of haematein is quite enough. 



257. BURCHABDT'S Pyroligneous Acid Haematoxylin (Arcli.w/l: 

 Anat., liii, 1898, p. 232) would seem to be superfluous at least. 



258. UNNA'S Oxidised Hsematoxylm (from MARTINOTTI, Zeit. 

 wiss. Mik., xxvii, 1910, p. 31). Haematoxylin 0'5, alum 2, 

 water 60, alcohol 10, glycerin 20, peroxide of hydrogen 

 solution 10, carbonate of soda.0'05. 



MARTINOTTI, loc. cit., makes it up with hsematein (0'2 grms). 



259. APATHY'S Haematein Mixture I A (Milth. Zool. Stat. 

 Neapel, xii, 1897, p. 712). Make (A) a solution of 9 per cent, 

 alum, 3 per cent, glacial acetic acid, and O'l per cent, 

 salicylic acid in water, and (B) a 1 per cent, solution of 

 haematoxylin in 70 per cent, alcohol, preserved for six to eight 

 weeks in a bottle not quite full. Mix one part of A with one 

 of B and one of glycerin. Stains either sections or material 

 in bulk. Apathy uses it for staining neuro-fibrils. 



260. KLEINENBERG'S Haematoxylin (Q-unrt. Journ. Micr. Sci., Ixxiv, 

 p -J08). Highly irmtioital and very incoushmt in its composition 



