H/EMATEIN (H7EMATOXYLIN) STAINS. 167 



269. Vanadium Haematoxylin (HEIDENHAIN, Encyclop. mik. 

 Tecknik., 1903, p. 518). Add 60 c.c. of a 6 per cent, solution of hsema- 

 toxylin to a 0'25 per cent, solution of vanadate of ammonium (quantity 

 not stated; should be 30 c.c., see COHN in Anat. Hefte, xv, 1895, p. 302). 

 The mixture to be used after three or four days ; it will not keep over 

 eight days. To be used with sections of sublimate material. A strong 

 plasma stain for special purposes, especially mucus glands. 



270. BENDA'S Copper Haematoxylin (Arch. mik. Anat., xxx, 1887, 

 p. 49). See fourth edition. According to my experience, not to be 

 compared with iron hajmatoxylin, and superfluous. 



271. MALLORY'S Pkospho-molybdic Acid Haematoxylin (Anat. 

 Anz., 1891, p. 375). One part 10 per cent, phosphomolybdic 

 jicid solution, 1 part hsematoxylin, 100 parts water, and 6 to' 

 10 parts chloral hydrate. Let the solution ripen for a week 

 in sunlight, and filter. Chiefly for central nervous system. 

 Sections should be stained for from ten minutes to one hour, 

 and washed out in two or three changes of 40 to 50 per cent, 

 alcohol. It is necessary that the solution should be saturated 

 with hgematoxylin in order to obtain the best results ; if a 

 good stain be not obtained at once, more ha3matoxylin must 

 be added. Water must never be used for diluting it. 



See also RIBBERT (Centralb. allg. Path., vii, 1896, p. 427 ; Zeit. wiss. 

 Mik., xv, 1898, p. 93), PATELLANI (Man. Zool. Ital., xiii, 1902, p. 6), and 

 GOLOVIN (Zeit. wiss. Mik., xix, 1902, p. 184). 



SARGENT (Anat. Anz., xv, 1898, p. 214) quotes this stain, preceded by 

 mordanting for twenty-four hours in 5 per cent, sulphate of copper, as 

 KENYON'S. 



KODIS (Arch. mik. Anat., lix, 1901, p. 211) takes hamiatoxylin, 1 part ; 

 niolybdic anhydride, 1*5 ; water, 100 ; H 2 O 2 , 0'5, or a crystal of HgO. 



POLICE (Arch. Zool. Napoli, iv, 1909, p. 300) takes 0'35 grin, haeina- 

 toxylin, 10 drops phospho-niolybdic acid of 10 per cent., 10 grms. chloral 

 hydrate, and 100 grms. alcohol of 70 per cent. 



272. MALLORY'S Phospho-tungstic Hsematoxylm (Journ. Exp. 

 Met!., v, 1900, p. 19 ; Zeit. wiss. Mik., xviii, 1901, p. 178) : 



Hsematoxylin . . . . .0*1 



Water 80'0 



10 per cent, solution of (MERCK'S) phospho- 



tungstic acid ..... 20'0 

 Peroxide of hydrogen (U.S. Ph.) . . 0'2 



(Dissolve the hasmatoxylin, add the acid, then the peroxide.) 

 Stain sections two to twenty-four hours, wash out with water. 



