168 OHAPTBB XIII. 



A poly chromic stain, nuclei blue, intercellular substances pink. 

 I consider tins a fine stain. 



273. DONAGGIO'S Tin Hsematoxylin (Ann. Ncvrol. Napoli, xxii, 

 1904, p. 192). A 1 per cent, solution of hamiatoxylin is poured slowly 

 into an equal volume of 20 per cent, solution of pink-salt (ammonio- 

 chloride of tin). Keep in the dark. 



274. Osmium Hsematoxylin. SCHULTZE (Zeit. wits. Milt., xxvii, 

 1910, p. 465) treats tissues for twenty-four hours or more with osmic 

 acid of 1 per cent., washes well with water, and puts for a couple of 

 days into ripened 0'5 per cent, solution of hamiatoxylin in alcohol of 35 

 to 50 per cent. Wash out for a day or more with alcohol of 70 per cent. 

 Intense plasma stain. 



