PLASMA STAINS WITH COAL-TAR DYES. 193 



Eosin is a specific stain for red blood-corpuscles, and also 

 for certain granules of leucocytes (see under " Blood "). 



The yolk of some ova takes the stain strongly, so that it 

 is useful in some embryological researches. 



311. EHRLICH'S Indulin-Aurantia-Eosin, or Acidophilous 

 Mixture, or Mixture C, or Mixture for Eosinophilous Cells (from 

 the formula kindly sent me by Dr. GRUELER). Indulin, 

 aurantia, and eosin, of each two parts ; glycerin, thirty 

 parts. This gives a very thick, syrupy solution. To use it, 

 cover-glass preparations may be floated on to it ; or sections 

 on slides may have a few drops poured on to them, the slide 

 being laid flat till the stain has taken effect (twenty-four hours 

 for Flemming material) . I find that with Flemming material 

 it gives a powerful and go.od stain, which is much more 

 resistant to alcohol than that of the EHRLICH-BIONDI mixture, 

 and is, therefore, much more adapted to ordinary work. 

 The stain keeps well. 



ISRAEL (Praktik. Path. Hist., Berlin, 1893, p. 68) gives a more 

 complicated receipt. 



312. Methyl Green and Eosin (CALBERLA, Morpli. Jahrb., iii, 1877, 

 Heft 3, p. 625 ; LIST, Zeit. wiss. Mik., ii, 1885, p. 147 ; BALBIANI, Ann. 

 Microgr., Paris, vii, 1895, p. 245 ; RHUMBLER, Zeit. wiss. Zool.,lxi, 1895, 

 p. 38). See early editions. 



313. Methylen Blue and Eosin (CHENZINSKY, quoted from Zeit. 

 wiss. Mile., xi, 2, 1894, p. 269). 



Methylen blue, sol. sat. in water . . . 40 



Eosin, 0'5 per cent, in 70 per cent, alcohol . . 20 

 Distilled water, or glycerin . . . . . .40 



This solution will only keep for about eight days. 

 PIANESE (ibid., xi, 1894, p. 345) adds a considerable proportion of 

 carbonate of lithia. 



See also the mixture of BREMER (Arch. mik. Anat., xlv, 1895, p. 446). 

 I have tried CHENZINSKY'S mixture as a tissue stain, without good 

 results ; but see ROSIN, Berliner Idin. Wochenschr., 1898, p. 251 ; Zeit. 

 wiss. Mik., xvi, 1899, p. 223, and xvii, 1900, p. 333. 



See also LAURENT (Centralb. allg. Path., xi, 1900, p. 86 ; Zeit. wiss. Mik., 

 xvii, 1900, p. 201). 



314. MALLORY'S Eosin and Methylen Blue (Journ. med. Research, 

 January, 1904). Sections of ZENKER material (other sublimate material 

 not so good) are stained for half to three quarters of an hour at 56 C. 

 in 5 per cent, aqueous solution of eosin, rinsed and flooded with solution 



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