196 CHAPTER XV. 



BAUMGAKTEN and JACOBY stain sections in a O2 per cent, 

 alcoholic solution. 



TONKOFF (Arch. mik. Anat., Ivi, 1900, p. 394) adds a little 

 tincture of iodine to the solution of the dye, or mordants 

 the sections with iodine. 



SKROBANSKY (Intern. Monatsschr. Anat., xxi, 1904, p. 20) 

 uses it in water with picric acid. 



327. Carmine Blue (Bleu Carmin Aqueux, from Meister, Lucius, 

 and Brunig, at Hochst-a-M.). JANSSENS (La Cellule, ix, 1893, p. 9) 

 states that this colour possesses a special affinity for the parts of cyto- 

 plasm that are undergoing cuticular differentiation. He uses it in 

 alcoholic solution acidified. 



328. Methyl Blue. Under this title are comprised some 

 other derivatives of the base rosanilin. They are "acid" 

 colours. Here belong Methyl Blue, Cotton Blue, Water Blue 

 (Wasserblau), Methyl Water-Blue, China Blue (Chinablau), 

 Soluble Blue. 



Amongst these Water Blue (Wasserblau) possesses some 

 useful properties. According to MITROPHANOW (quoted from 

 Zeit. wiss. MiJc., v, 188, p. 513), used in concentrated 

 aqueous solution it gives a very good double stain with 

 safranin. It is very resistant to alcohol. Using the Wasser- 

 blau first, and then the safranin, I have had some interesting 

 results. The Wasserblau must be used first. With chrom- 

 osmium material, twelve to twenty-four hours in the blue, 

 and four or five in the safranin, may not be too much. My 

 stains have not kept well. 



MANN (Methods, etc., p. 216) uses a mixture of 35 parts 

 1 per cent, solution of eosin, 45 of methyl blue, and 100 of 

 water. He has also (Zeit. wiss. Mik., xi, 1894, p. 490) used 

 a similar mixture for nerve-cells. 



329. Anilin Blue-black. A preparation cited under this name has 

 been recommended by BEVAN LEWIS and others for nervous tissue. 

 The dye used by them cannot now be identified. Dr. GBUBLER writes 

 me that the anilin blue-black of his list is the oxyazo colour blue- 

 black B or AZOSCHWABZ ; but that dye had not been discovered when 

 Bevan Lewis wrote. See also HEIDENHAIN in Zeit. wiss. Mile., xx, 

 1903, p. 185, and xxv, 1909, p. 407. 



330. Violet B (or Methyl Violet B) (S. MAYER, Sifzl. k. k. 

 AkatL ?r/.s-.v. ll'len, iii Abth, February, 1882). Used in 

 solutions of 1 grm. of the colour to 300 grms. of 0'5 per cent. 



